CAJ | 학술논문

类球红细菌（Rhodobacter sphaeroides）作为微生态制剂的生产菌种之一,具有广泛的应用价值。甘薯淀粉废水是不含有毒物质的高浓度有机废水,能够提供类球红细菌生长所需的营养物质。单因素实验表明,培养因素中甘薯淀粉废水的浓度、pH和温度为主要影响因子。正交试验优化后最佳培养条件为：甘薯淀粉废水稀释度60%,pH值7.0、温度35℃、接种量7.5%、转速200 r/min,此条件下,24 h类球红细菌浓度达6.7×109CFU/mL,与LB培养液（6.9×109CFU/mL）无显著差异,表明用甘薯淀粉废水培养类球红细菌是一种废水资源化利用的可行途径。
류구홍세균（Rhodobacter sphaeroides）작위미생태제제적생산균충지일,구유엄범적응용개치。감서정분폐수시불함유독물질적고농도유궤폐수,능구제공류구홍세균생장소수적영양물질。단인소실험표명,배양인소중감서정분폐수적농도、pH화온도위주요영향인자。정교시험우화후최가배양조건위：감서정분폐수희석도60%,pH치7.0、온도35℃、접충량7.5%、전속200 r/min,차조건하,24 h류구홍세균농도체6.7×109CFU/mL,여LB배양액（6.9×109CFU/mL）무현저차이,표명용감서정분폐수배양류구홍세균시일충폐수자원화이용적가행도경。
As one of probiotics strains, Rhodobacter sphaeroides has extensive application value. Sweet potato starch wastewater is high concentration organic wastewater without toxic substances and can provides nutrients for Rhodobacter spha- eroides growth. Single factor experiment shows that the concentration, pH and temperature of sweet potato wastewater are the main influence factors. Orthogonal experiment shows the optimum culture conditions of Rhodobacter sphaeroides： the wastewater was diluted to 60%, pH 7.0, temperature 35℃, illumination 300 lx, inoculum 7.5%, rotate speed 200 r/min. After 24 h cultivation, Rhodobacter sphaeroides cells reached to 6.7 ×10^9 CFU/mL, which showed no significant difference comparing to LB culture medium. Result Indicates it is feasible to ferment Rhodobacter sphaeroides using sweet potato starch wastewater.