CAJ | 학술논문

目的：探讨急性心肌缺血再灌注大鼠心功能的改变及心肌纤维化对其影响。方法：建立大鼠急性心肌缺血再灌注模型，利用心脏超声和血流动力学检测其心功能状态。用水解法检测心肌羟脯氨酸含量。分别用免疫印迹和酶联免疫吸附剂测定法检测心肌和血清中 TNF-α的表达。结果：急性心肌缺血再灌注大鼠早期出现心脏舒张功能降低，在灌注第3天时尤为显著。心肌纤维化发生在缺血再灌注区，且该部位胶原容积分数显著增高，与心肌TNF-α表达规律相同。同时心肌羟脯氨酸含量和血清TNF-α在急性缺血再灌注时也显著增加。结论：急性心肌缺血再灌注大鼠心肌纤维化降低早期心脏舒张功能，而炎症因子TNF-α的异常增高是急性心肌缺血再灌注心肌纤维化的可能因素之一。
목적：탐토급성심기결혈재관주대서심공능적개변급심기섬유화대기영향。방법：건립대서급성심기결혈재관주모형，이용심장초성화혈류동역학검측기심공능상태。용수해법검측심기간포안산함량。분별용면역인적화매련면역흡부제측정법검측심기화혈청중 TNF-α적표체。결과：급성심기결혈재관주대서조기출현심장서장공능강저，재관주제3천시우위현저。심기섬유화발생재결혈재관주구，차해부위효원용적분수현저증고，여심기TNF-α표체규률상동。동시심기간포안산함량화혈청TNF-α재급성결혈재관주시야현저증가。결론：급성심기결혈재관주대서심기섬유화강저조기심장서장공능，이염증인자TNF-α적이상증고시급성심기결혈재관주심기섬유화적가능인소지일。
Objective:To discuss the cardiac function changes and the myocardial fibrosis effects in acute myocardial ischemia/reperfusion rats .Methods :Acute myocardial ischemia/reperfusion rats models were estab-lished for detecting their heart function by echocardiography and hemodynamic .Water solution method was used to detect expression of myocardial hydroxyproline .And the expression of TNF-αin the myocardial and serum was de-tected by western blot and enzyme-linked immunosorbent method .Results :Early heart diastolic function was lower in acute myocardial ischemia/reperfusion rat ,significantly in the third day after perfusion .Myocardial fibrosis oc-curred in ischemia/reperfusion areas ,and the collagen volume fraction was significantly increased in this area .It was identical to expression of TNF-αin myocardial .Meanwhile ,myocardial hydroxyproline content and TNF-αin serum also significantly increased in acute ischemia/reperfusion .Conclusion:Myocardial fibrosis results to deterioration of early heart diastolic functionin in rats with acute myocardial ischemia/reperfusion .And the expression of inflamma-tory cytokine TNF-αis one of the possible factors of myocardial fibrosis in acute myocardial ischemia/reperfusion .