CAJ | 학술논문

对收集的24个芦笋品种进行ISSR分析。结果表明：共计获得98个扩增位点，多态性位点74个，占总扩增位点数的75．61％。每条引物平均扩增位点数为10个。采用NTSYS2．1软件进行相似系数分析，建立聚类图．在阈值为0．771可以把中国、日本、意大利和美国等4个国家的品种区分开来，但是荷兰和美国的品种却聚为一类。主成分分析结果与相似系数分析结果基本上一致。
대수집적24개호순품충진행ISSR분석。결과표명：공계획득98개확증위점，다태성위점74개，점총확증위점수적75．61％。매조인물평균확증위점수위10개。채용NTSYS2．1연건진행상사계수분석，건립취류도．재역치위0．771가이파중국、일본、의대리화미국등4개국가적품충구분개래，단시하란화미국적품충각취위일류。주성분분석결과여상사계수분석결과기본상일치。
Twenty-four asparagus germplasms as experimental materials were analyzed by using inter-simple sequence repeat （ISSR）, and the results showed that 98 fragments of DNA were amplified with 10 primers by using ISSR-PCR, among which 74 （75.61%） fragments were polymorphic. Each primer amplified 10 fragments of DNA. The genetic similarity coefficient was analyzed, and a tree diagram constructed by using UPGMA algorithm. According to this tree diagram four countries varieties form China, Japan, Italy and America were distinguished, but the Netherlands and the USA cultivars were constructed one group; and the results of the principal component analysis and the similarity coefficient analysis are basically consistent.