饲料博览
飼料博覽
사료박람
FEED PANORAMA
2012年
3期
5-9
,共5页
刘丽琼%聂奎%余姣%廖鑫凯
劉麗瓊%聶奎%餘姣%廖鑫凱
류려경%섭규%여교%료흠개
小鼠%急性结肠炎%TLRs/MyD88通路%mRNA表达量%荧光定量RT-PCR
小鼠%急性結腸炎%TLRs/MyD88通路%mRNA錶達量%熒光定量RT-PCR
소서%급성결장염%TLRs/MyD88통로%mRNA표체량%형광정량RT-PCR
mice%acute colitis%TLRs/MyD88 pathway%mRNA expression%real-time RT-PCR
试验旨在研究TLR2、4/MyD88通路相关分子mRNA在炎症过程中的作用机制及相互关系。诱导急性结肠炎小鼠模型,分别于造模前、造模后1、3、5、7 d各处死6只小鼠,并采用实时荧光定量RT-PCR检测结肠组织中TLR2、TLR4、MyD88、NF-κB、TNF-α和IL-10 mRNA的表达量变化。结果表明,与造模前比较,TLR2mRNA在造模后3、5 d的表达量显著增加（P〈0.05）,造模后7 d时的表达具有极显著差异（P〈0.001）;TLR4在造模后1 d就显著上调（P〈0.05）,在造模后3、5、7 d更是异常高表达（P〈0.001）;MyD88和NF-κB mRNA的表达量都在造模后5、7 d迅速增加（P〈0.01）;TNF-α在造模后各阶段（除造模后1 d外）均出现高表达（P〈0.01或P〈0.001）;IL-10在造模后1 d的转录水平急剧上升（P〈0.001）,此后则逐渐回落,但其表达水平高于造模前。TLR2/4可能通过正调节MyD88、NF-κB和TNF-α,负调节IL-10等关键节点分子的mRNA表达水平来参与炎症反应。
試驗旨在研究TLR2、4/MyD88通路相關分子mRNA在炎癥過程中的作用機製及相互關繫。誘導急性結腸炎小鼠模型,分彆于造模前、造模後1、3、5、7 d各處死6隻小鼠,併採用實時熒光定量RT-PCR檢測結腸組織中TLR2、TLR4、MyD88、NF-κB、TNF-α和IL-10 mRNA的錶達量變化。結果錶明,與造模前比較,TLR2mRNA在造模後3、5 d的錶達量顯著增加（P〈0.05）,造模後7 d時的錶達具有極顯著差異（P〈0.001）;TLR4在造模後1 d就顯著上調（P〈0.05）,在造模後3、5、7 d更是異常高錶達（P〈0.001）;MyD88和NF-κB mRNA的錶達量都在造模後5、7 d迅速增加（P〈0.01）;TNF-α在造模後各階段（除造模後1 d外）均齣現高錶達（P〈0.01或P〈0.001）;IL-10在造模後1 d的轉錄水平急劇上升（P〈0.001）,此後則逐漸迴落,但其錶達水平高于造模前。TLR2/4可能通過正調節MyD88、NF-κB和TNF-α,負調節IL-10等關鍵節點分子的mRNA錶達水平來參與炎癥反應。
시험지재연구TLR2、4/MyD88통로상관분자mRNA재염증과정중적작용궤제급상호관계。유도급성결장염소서모형,분별우조모전、조모후1、3、5、7 d각처사6지소서,병채용실시형광정량RT-PCR검측결장조직중TLR2、TLR4、MyD88、NF-κB、TNF-α화IL-10 mRNA적표체량변화。결과표명,여조모전비교,TLR2mRNA재조모후3、5 d적표체량현저증가（P〈0.05）,조모후7 d시적표체구유겁현저차이（P〈0.001）;TLR4재조모후1 d취현저상조（P〈0.05）,재조모후3、5、7 d경시이상고표체（P〈0.001）;MyD88화NF-κB mRNA적표체량도재조모후5、7 d신속증가（P〈0.01）;TNF-α재조모후각계단（제조모후1 d외）균출현고표체（P〈0.01혹P〈0.001）;IL-10재조모후1 d적전록수평급극상승（P〈0.001）,차후칙축점회락,단기표체수평고우조모전。TLR2/4가능통과정조절MyD88、NF-κB화TNF-α,부조절IL-10등관건절점분자적mRNA표체수평래삼여염증반응。
To study the association of TLR2,4/MyD88 pathway molecules mRNA in inflammation,a mouse model with acute colitis was induced.Six mice were killed respectively for 0,1,3,5 and 7 day old.The mRNA expression levels of TLR2,TLR4,MyD88,NF-κB,TNF-α and IL-10 were detected by quantitative real-time reverse-transcriptase polymerase chain reaction.The results showed that,compared with the 0 day group,the transcription level of TLR2 mRNA was significantly high on 3,5 days（P〈0.05）,and its expression was a significantly difference on 3,5 day old（P〈0.001）;1 day old after were induced,the expression of TLR4 was up-regulated significantly（P〈0.05）,and it was more abnormal expression on 3,5 and 7 day old（P〈0.001）;MyD88 and NF-κB mRNA were increased rapidly on 5,7 day old（P0.01）;it showned that TNF-α was high expression at all stages（excepting one day old）（P0.01 or P〈0.001）;the transcription level of IL-10 rised sharply in the first day（P〈0.001）,since then it reduced gradually but its expression level were still higher than the previous of induction.TLR2/4 possibly through up-regulate mRNA expressions of MyD88,NF-κB and TNF-α,but down-regulate the transcription level of IL-10 mRNA to contribute to the inflammatory response.
