CAJ | 학술논문

将质粒pcDNA3．1-VEGF双酶切后亚克隆至逆转录病毒载体pLXSN，并用酶切及测序等手段进行鉴定．而后利用脂质体转染包装细胞pA317，并检测病毒滴度．结果表明：成功构建了VEGF基因的重组逆转录病毒表达载体．
장질립pcDNA3．1-VEGF쌍매절후아극륭지역전록병독재체pLXSN，병용매절급측서등수단진행감정．이후이용지질체전염포장세포pA317，병검측병독적도．결과표명：성공구건료VEGF기인적중조역전록병독표체재체．
According to compatible restriction sites, we digest the plasmid pcDNA3.1 -VEGF and pLXSN and connect them together,then perform enzyme cutting and sequence analysis to identify the connection. Lipofeetamine was Used to me- diate recombinant plasmid pLXSN - VEGF transfection of pA317 packaging cells, collect virus - containing medium from packaging cells, infect NIH3T3 cells to determine viral titer. The results indicate that retroviral vector pLXSN- VEGF was successfully established.