CAJ | 학술논문

镰刀菌是玉米穗腐病的主要病原菌.本研究对南阳市5县2009年夏玉米穗腐病致病镰刀菌的种群结构进行调查分析.从病样中按常规组织分离法进行病原菌分离,对分离物经单孢分离纯化后根据柯赫法则进行致病性检测,并根据形态特征和核糖体基因内转录间隔区（rDNA-ITS）序列对病原菌进行鉴定.从采集的80份穗腐病样品中分离获得105株镰刀菌,接种结果表明,所有菌株对玉米均有致病性.形态和分子鉴定结果表明,引起南阳市玉米穗腐病的镰刀菌至少有4种：层出镰刀菌Fusarium proliferatum、串珠赤霉菌Gibberella monoliformis、木贼镰刀菌F.equiseti及玉蜀黍赤霉菌G.zeae.南阳市玉米穗腐病致病镰刀菌以层出镰刀菌为优势种,串珠赤霉菌为次优势种.
렴도균시옥미수부병적주요병원균.본연구대남양시5현2009년하옥미수부병치병렴도균적충군결구진행조사분석.종병양중안상규조직분리법진행병원균분리,대분리물경단포분리순화후근거가혁법칙진행치병성검측,병근거형태특정화핵당체기인내전록간격구（rDNA-ITS）서렬대병원균진행감정.종채집적80빈수부병양품중분리획득105주렴도균,접충결과표명,소유균주대옥미균유치병성.형태화분자감정결과표명,인기남양시옥미수부병적렴도균지소유4충：층출렴도균Fusarium proliferatum、천주적매균Gibberella monoliformis、목적렴도균F.equiseti급옥촉서적매균G.zeae.남양시옥미수부병치병렴도균이층출렴도균위우세충,천주적매균위차우세충.
Fusarium is the main causal agent of maize ear rot.The population structure of Fusarium species causing maize ear rot in 5 counties of Nanyang City was investigated.The pathogens were isolated from diseased corn samples using conventional tissue isolation method.The pathogenicity of the single spore derived isolates on corn leaves and ears was tested based on Koch＇s postulates.The pathogenic isolates were identified based on morphological characteristics and rDNA-ITS（internal transcribed spacer） sequences.Totally 105 Fusarium isolates were obtained from 80 maize ear rot samples collected from the investigation areas.The inoculation tests showed that all the Fusarium isolates obtained were virulent on corn leaves and ears.At least 4 fungal species were identified as causal agents of maize ear rot based on their morphological characteristics and rDNA-ITS sequences.They were Fusarium proliferatum,Gibberella monoliformis,F.equiseti and G.zeae.F.proliferatum and G.moniliformis were found to be the predominant and subdominant species,respectively,among the Fusarium spp.and Gibberella spp.associated with maize ear rot in Nanyang City.