现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
6期
23-24
,共2页
3-甲基-2-乙酰基-喹喔啉%N4-脱一氧乙酰甲喹%薄层扫描法
3-甲基-2-乙酰基-喹喔啉%N4-脫一氧乙酰甲喹%薄層掃描法
3-갑기-2-을선기-규악람%N4-탈일양을선갑규%박층소묘법
3-methyl-2-acetyl-quinoxaline%4-desoxymequindox%thin layer chromatograph
采用薄层扫描法同时测定N4-脱一氧乙酰甲喹及3-甲基-2-乙酰基-喹喔啉（脱二氧乙酰甲喹）的含量。试验采用甲醇为溶剂对N4-脱一氧乙酰甲喹及脱二氧乙酰甲喹进行溶解,以石油醚-乙酸乙酯（6∶1）为展开剂上行展开;以瑞士卡玛IV型扫描仪,采用单波长吸收扫描法,λ=254 nm。结果表明：以扫描所得峰面积积分计算,N4-脱一氧乙酰甲喹及脱二氧乙酰甲喹的线性范围均为0.092～0.736μg（r≥0.985）。该方法适用于大规模样品中N4-脱一氧乙酰甲喹及脱二氧乙酰甲喹含量初筛。
採用薄層掃描法同時測定N4-脫一氧乙酰甲喹及3-甲基-2-乙酰基-喹喔啉（脫二氧乙酰甲喹）的含量。試驗採用甲醇為溶劑對N4-脫一氧乙酰甲喹及脫二氧乙酰甲喹進行溶解,以石油醚-乙痠乙酯（6∶1）為展開劑上行展開;以瑞士卡瑪IV型掃描儀,採用單波長吸收掃描法,λ=254 nm。結果錶明：以掃描所得峰麵積積分計算,N4-脫一氧乙酰甲喹及脫二氧乙酰甲喹的線性範圍均為0.092～0.736μg（r≥0.985）。該方法適用于大規模樣品中N4-脫一氧乙酰甲喹及脫二氧乙酰甲喹含量初篩。
채용박층소묘법동시측정N4-탈일양을선갑규급3-갑기-2-을선기-규악람（탈이양을선갑규）적함량。시험채용갑순위용제대N4-탈일양을선갑규급탈이양을선갑규진행용해,이석유미-을산을지（6∶1）위전개제상행전개;이서사잡마IV형소묘의,채용단파장흡수소묘법,λ=254 nm。결과표명：이소묘소득봉면적적분계산,N4-탈일양을선갑규급탈이양을선갑규적선성범위균위0.092～0.736μg（r≥0.985）。해방법괄용우대규모양품중N4-탈일양을선갑규급탈이양을선갑규함량초사。
The paper determined simultaneously 3-methyl-2-acetyl-quinoxaline and 4-desoxymequindox.Methanol was used as solvent.Linear ascending development was carried out with petroleum ether：ethyl acetate（6∶1）at room temperature.Camag TLC scanner IV was used for spectrodensitometric scanning and analysis in absorbance mode at 254 nm.The results showed that the data for calibration plots showed good linear relationship with r≥0.985 in the concentration range of 0.092 μg to 0.736 μg with respect to peak area.This method was suitable for screening large-scale sample containing 3-methyl-2-acetyl-quinoxaline and 4-desoxymequindox.
