CAJ | 학술논문

采用pH=6.0的NaAc-HAc缓冲液直接提取化妆品中氟喹诺酮类（氧氟沙星、诺氟沙星和环丙沙星）抗生素,建立反相高效液相色谱同时测定化妆品中氧氟沙星、诺氟沙星和环丙沙星的方法。以十八烷基硅烷键合硅胶为填充剂,以0.05 mol/L柠檬酸与1.0 mol/L醋酸铵（77∶1,用三乙胺调节pH至4.0）混合液——乙腈（85∶15）为流动相,检测波长为280 nm的条件下进行测定。结果表明氧氟沙星、诺氟沙星和环丙沙星的响应线性良好,回收率高。
채용pH=6.0적NaAc-HAc완충액직접제취화장품중불규낙동류（양불사성、낙불사성화배병사성）항생소,건립반상고효액상색보동시측정화장품중양불사성、낙불사성화배병사성적방법。이십팔완기규완건합규효위전충제,이0.05 mol/L저몽산여1.0 mol/L작산안（77∶1,용삼을알조절pH지4.0）혼합액——을정（85∶15）위류동상,검측파장위280 nm적조건하진행측정。결과표명양불사성、낙불사성화배병사성적향응선성량호,회수솔고。
A HPLC method was established for the assay of fluoroqronolone antibiotics（ofloxacin,norfloxacin and ciprofloxacin）,which were extracted directly with the buffer of NaAc-HAc（pH 6.0）in cosmetics.The sample was analyzed on C18 with the buffer-acetonitrile（85：15）,which was consisted of 0.05 mol/L citric acid-acetic ammonium（77：1） and adjusting to pH 4.0 with triethanolamine,as the mobile phase and 280 nm as the detection wavelength.This method was proved to be highly effective and practicable.