广东化工
廣東化工
엄동화공
GUANGDONG CHEMICAL INDUSTRY
2012年
4期
87-88,90
,共3页
酶水解%发酵%冰糖橙皮渣%乙醇%工艺
酶水解%髮酵%冰糖橙皮渣%乙醇%工藝
매수해%발효%빙당등피사%을순%공예
enzyme-hydrolyze%ferment%bingtang sweet orange peel waste%ethanol%technology
采用纤维素酶、果胶酶和β-葡萄糖苷酶对冰糖橙皮渣进行水解,所得还原糖液接种异常毕赤酵母进行发酵,考察了酵母接种量、发酵时间、pH和发酵温度等单因素对乙醇得率的影响。单因素结果表明：接种量为12%、发酵时间72 h、pH 4.5、发酵温度33℃时乙醇得率最高。在此基础上设计L9（34）正交实验。结果表明,最佳工艺条件为pH 4.5,接种量12%,发酵时间72 h,发酵温度30℃。在此条件下乙醇产率为0.2451 g/g,显著高于单因素实验（0.2263 g/g）和正交实验结果（0.2329 g/g）。
採用纖維素酶、果膠酶和β-葡萄糖苷酶對冰糖橙皮渣進行水解,所得還原糖液接種異常畢赤酵母進行髮酵,攷察瞭酵母接種量、髮酵時間、pH和髮酵溫度等單因素對乙醇得率的影響。單因素結果錶明：接種量為12%、髮酵時間72 h、pH 4.5、髮酵溫度33℃時乙醇得率最高。在此基礎上設計L9（34）正交實驗。結果錶明,最佳工藝條件為pH 4.5,接種量12%,髮酵時間72 h,髮酵溫度30℃。在此條件下乙醇產率為0.2451 g/g,顯著高于單因素實驗（0.2263 g/g）和正交實驗結果（0.2329 g/g）。
채용섬유소매、과효매화β-포도당감매대빙당등피사진행수해,소득환원당액접충이상필적효모진행발효,고찰료효모접충량、발효시간、pH화발효온도등단인소대을순득솔적영향。단인소결과표명：접충량위12%、발효시간72 h、pH 4.5、발효온도33℃시을순득솔최고。재차기출상설계L9（34）정교실험。결과표명,최가공예조건위pH 4.5,접충량12%,발효시간72 h,발효온도30℃。재차조건하을순산솔위0.2451 g/g,현저고우단인소실험（0.2263 g/g）화정교실험결과（0.2329 g/g）。
The Bingtang sweet orange peel waste was hydrolyzed by celluase,pectinase and β-glucosidase.The resulting reducing sugar solution was inoculated with Pichia anomala and fermented.The effects of single factors including yeast inoculums,fermentation time,pH and fermentation temperature on ethanol yield were illustrated.Results showed that the optimum condition for single factor experiments were as follows： inoculums 12 %;fermentation time 72 h;pH 4.5;fermentation temperature 33 ℃.Then a L9（34） orthogonal test was designed based on the forgoing results.Results showed the optimal fermentation technology was pH 4.5;inoculums 12 %;fermentation time 72 h;fermentation temperature 30 ℃.Under these conditions,the ethanol yield could reach 0.2451 g/g,significant higher than those in single factor experiment（0.2263 g/g） and orthodox experiments（0.2329 g/g）.
