发酵科技通讯
髮酵科技通訊
발효과기통신
FAXIAO KEJI TONGXUN
2012年
2期
16-20
,共5页
里氏木霉%少孢根霉协联糖化
裏氏木黴%少孢根黴協聯糖化
리씨목매%소포근매협련당화
Trichoderma reesei%Rhizopus oligosporus%Coupling%Saccharification
摘要：本文以双酶协联糖化技术处理粉渣中纤维素，并利用单因素和正交试验考察菌种混合比例、接种量，协联糖化温度与协联糖化时间对纤维素糖化率的影响，结果表明，双霉协联糖化的最优条件为：少孢根霉与里氏木霉混合比值为1．5，接种量为1．8×10^8个孢子／ml，协联糖化温度为34℃，协联糖化时间为72h，在此工艺条件下纤维素糖化率高达82．3％。纤维素酶活力达到645．5U／ml-664U／m1．与传统单菌种发酵的糖化技术相比，提高了160U／ml-280U／ml。
摘要：本文以雙酶協聯糖化技術處理粉渣中纖維素，併利用單因素和正交試驗攷察菌種混閤比例、接種量，協聯糖化溫度與協聯糖化時間對纖維素糖化率的影響，結果錶明，雙黴協聯糖化的最優條件為：少孢根黴與裏氏木黴混閤比值為1．5，接種量為1．8×10^8箇孢子／ml，協聯糖化溫度為34℃，協聯糖化時間為72h，在此工藝條件下纖維素糖化率高達82．3％。纖維素酶活力達到645．5U／ml-664U／m1．與傳統單菌種髮酵的糖化技術相比，提高瞭160U／ml-280U／ml。
적요：본문이쌍매협련당화기술처리분사중섬유소，병이용단인소화정교시험고찰균충혼합비례、접충량，협련당화온도여협련당화시간대섬유소당화솔적영향，결과표명，쌍매협련당화적최우조건위：소포근매여리씨목매혼합비치위1．5，접충량위1．8×10^8개포자／ml，협련당화온도위34℃，협련당화시간위72h，재차공예조건하섬유소당화솔고체82．3％。섬유소매활력체도645．5U／ml-664U／m1．여전통단균충발효적당화기술상비，제고료160U／ml-280U／ml。
The optimization conditions on saccharification of cellulose coupling with Rhizopu- soligosporus and Trichoderma reesei were researched. Using the method of single factor and orthogonal, mix- ing ratio of strains, inoculum, saccharification temperature and saccharification time were determined on the effect of saecharification rate of cellulose. The optimums aeeharifieation conditions were showed that the mixing ratio of Rhizopus oligosporus and Trichoderma reesei 1.5, the inoculum 1.8Хl08spores/ml, the sacchar- ification temperature 34℃,the saeeharification time 72h. In this condition, the rate of saccharification of cel- lulose was 82.3%.Cellulase activity was 645.SU/ml-664U/ml, compared with the traditional biology sacchar- ification with single strain, cellulase activity improved 160U/ml-280U/ml
