山东林业科技
山東林業科技
산동임업과기
JOURNAL OF SHANDONG FORESTRY SCIENCE AND TECHNOLOGY
2012年
2期
1-7
,共7页
宋新红%丰震%谷衍川%张鑫鑫%王长宪%张林
宋新紅%豐震%穀衍川%張鑫鑫%王長憲%張林
송신홍%봉진%곡연천%장흠흠%왕장헌%장림
紫薇%多倍体%诱导%倍性鉴定%形态比较%内多倍性
紫薇%多倍體%誘導%倍性鑒定%形態比較%內多倍性
자미%다배체%유도%배성감정%형태비교%내다배성
Lagerstroernia indica L.%polyploid%induction%ploidy determination%morphology comparison%endopolyploidy
以紫薇子叶期幼苗为试验材料,采用不同秋水仙素浓度和时间组合处理其生长点,成功获得四倍体(2n=4x=96)和六倍体(2n=6x=144)。诱变株与二倍体对照植株形态差异明显,植株矮小粗壮,叶片变皱、粗糙,增厚,气孔密度减小,气孔保卫细胞(一对,下同)长、宽分别增大65.79%和45.01%,保卫细胞内叶绿体数量增加80.25%,且颗粒增大。四倍体花粉粒直径增大59.07%,花瓣长宽略有增加,但花序长和花朵数反而减少。人工诱导四倍体和普通二倍体茎尖都有内多倍化现象,并且前者内多倍化水平低于后者。
以紫薇子葉期幼苗為試驗材料,採用不同鞦水仙素濃度和時間組閤處理其生長點,成功穫得四倍體(2n=4x=96)和六倍體(2n=6x=144)。誘變株與二倍體對照植株形態差異明顯,植株矮小粗壯,葉片變皺、粗糙,增厚,氣孔密度減小,氣孔保衛細胞(一對,下同)長、寬分彆增大65.79%和45.01%,保衛細胞內葉綠體數量增加80.25%,且顆粒增大。四倍體花粉粒直徑增大59.07%,花瓣長寬略有增加,但花序長和花朵數反而減少。人工誘導四倍體和普通二倍體莖尖都有內多倍化現象,併且前者內多倍化水平低于後者。
이자미자협기유묘위시험재료,채용불동추수선소농도화시간조합처리기생장점,성공획득사배체(2n=4x=96)화륙배체(2n=6x=144)。유변주여이배체대조식주형태차이명현,식주왜소조장,협편변추、조조,증후,기공밀도감소,기공보위세포(일대,하동)장、관분별증대65.79%화45.01%,보위세포내협록체수량증가80.25%,차과립증대。사배체화분립직경증대59.07%,화판장관략유증가,단화서장화화타수반이감소。인공유도사배체화보통이배체경첨도유내다배화현상,병차전자내다배화수평저우후자。
Tetraploid (2n=4x= 96) and hexaploid (2n= 6x= 144) plants were successfully induced from seedling of Lagerstroernia indica L. by processing shoot apical meristem with colchicine. The results indicated that the variants, which were characterized by stunted but stout plant, wrinkled and thicker leaves, less stoma frequency, larger(length rised by 65.79 % and width, 45.01%) guard cells (a pair, the same as below), more larger(rised by 80.25%) chloroplasts in each pair of guard cells, were significantly different from the diploid plants in morphology. Tetraploid plant got markedly larger (rised by 59. 07%) pollen grains, slightly larger petals, longer inflorescence but less flowers than diploid plants. Endopolyploidy was observed in both induced tetraploid plant and diploid plant, and endopolyploidy degree of the former was lower than that of the latter.