CAJ | 학술논문

目的：建立浊度法测定酒石酸泰乐菌素可溶性粉效价的方法。方法：以金黄色葡萄球菌为试验菌,抗生素检定培养基Ⅲ号为试验用培养基,制备菌悬液。采用浊度法和管碟法对酒石酸泰乐菌素可溶性粉的效价测定进行比较,并对浊度法进行方法学验证。结果：用浊度法测定酒石酸泰乐菌素可溶性粉的效价,泰乐菌素的浓度范围在0.6-2.0 u/mL之间,其浓度对数与吸收度有良好的线性关系,相关系数r=0.999 0,平均回收率为100.3%,RSD为0.91%;用浊度法与管碟法测定结果无明显差异,方法学验证表明,浊度法测定酒石酸泰乐菌素可溶性粉效价的方法可行。结论：本方法具有可信限率低、灵敏度高、精密度高、快速简便等优点,可用于泰乐菌素及其制剂的效价测定。
목적：건립탁도법측정주석산태악균소가용성분효개적방법。방법：이금황색포도구균위시험균,항생소검정배양기Ⅲ호위시험용배양기,제비균현액。채용탁도법화관설법대주석산태악균소가용성분적효개측정진행비교,병대탁도법진행방법학험증。결과：용탁도법측정주석산태악균소가용성분적효개,태악균소적농도범위재0.6-2.0 u/mL지간,기농도대수여흡수도유량호적선성관계,상관계수r=0.999 0,평균회수솔위100.3%,RSD위0.91%;용탁도법여관설법측정결과무명현차이,방법학험증표명,탁도법측정주석산태악균소가용성분효개적방법가행。결론：본방법구유가신한솔저、령민도고、정밀도고、쾌속간편등우점,가용우태악균소급기제제적효개측정。
Objective： To establish a method for the determination of the titer of tartaric acid tylosin soluble powder.Methods： Using Staphylococcus aureus（SA） as the test organisms and antibiotic culture medium Ⅲ as the test culture medium,the titer of tartaric acid tylosin soluble powder was determined by cylinder-plate method and turbidimetric method.Results： The result demonstrated that the titer of tartaric acid tylosin soluble powder presented a good linear relation with its absorbance among the range of 0.6-2.0 u/mL.The results of two methods showed no difference.Conclusion： It is indicated that turbidimetric method was accurate,reliable and suitable for the determination of tartaric acid tylosin soluble powder.