畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2012年
5期
130-131
,共2页
猪繁殖与呼吸综合征%PRRSV%免疫抗体%抗体检测%散养猪
豬繁殖與呼吸綜閤徵%PRRSV%免疫抗體%抗體檢測%散養豬
저번식여호흡종합정%PRRSV%면역항체%항체검측%산양저
porcine reproductive and respiratory syndrome%porcine reproductive and respiratory syndrome virus%immune antibody%antibody detection%scatter-feed pigs
进行猪繁殖与呼吸综合征(PRRS)疫苗安全及效力试验时,需要选用猪繁殖与呼吸综合征病毒(PRRSV)抗原、抗体均为阴性的猪血清。为此,应用EUSA和RT—PCR方法对取自陕西省关中部分县(区)散养的未进行猪繁殖与呼吸综合征疫苗免疫猪群共计135份血清样品进行了PRRSV抗体和抗原测定。结果表明,PRRSV抗体阳性率为6.67%(9/135).PRRSV抗体阴性猪群中抗原阳性率为11.11%(14/126)。由此可以得出,该地区农户散养的猪群存在感染猪繁殖与呼吸综合征病毒的危险。
進行豬繁殖與呼吸綜閤徵(PRRS)疫苗安全及效力試驗時,需要選用豬繁殖與呼吸綜閤徵病毒(PRRSV)抗原、抗體均為陰性的豬血清。為此,應用EUSA和RT—PCR方法對取自陝西省關中部分縣(區)散養的未進行豬繁殖與呼吸綜閤徵疫苗免疫豬群共計135份血清樣品進行瞭PRRSV抗體和抗原測定。結果錶明,PRRSV抗體暘性率為6.67%(9/135).PRRSV抗體陰性豬群中抗原暘性率為11.11%(14/126)。由此可以得齣,該地區農戶散養的豬群存在感染豬繁殖與呼吸綜閤徵病毒的危險。
진행저번식여호흡종합정(PRRS)역묘안전급효력시험시,수요선용저번식여호흡종합정병독(PRRSV)항원、항체균위음성적저혈청。위차,응용EUSA화RT—PCR방법대취자합서성관중부분현(구)산양적미진행저번식여호흡종합정역묘면역저군공계135빈혈청양품진행료PRRSV항체화항원측정。결과표명,PRRSV항체양성솔위6.67%(9/135).PRRSV항체음성저군중항원양성솔위11.11%(14/126)。유차가이득출,해지구농호산양적저군존재감염저번식여호흡종합정병독적위험。
During the detection on safety and effectiveness of porcine reproductive and respiratory syndrome (PRRS) vaccine, pigs which antigen and antibodies of PRRS negative were needed. In this experiment, 135 pig serum samples which came from five counties (districts) of Guanzhong Area, Shaanxi Province were tested by ELISA and RT-PCR method. The results showed that antibody-positive rate and antigen positive rate of PRRS were 6.67% (9/135) and ll.ll% (14/126) respectively. It proved that the presentments and control of PRRS in scatter-feed herds were insufficient, there is great danger of PRRSV infection.