CAJ | 학술논문

目的：建立银翘解毒片特征图谱的测定方法，整体评价银翘解毒片的质量。方法采用色谱柱：Kromasil 100-5 c18(4.6 mm ×250 mm)；检测器：dad 检测器；检测波长：279nm；柱温：30℃；甲醇-0.2%磷酸为流动相，1ml·min-1的流速梯度洗脱。结果模拟银翘解毒片剂特征图谱有12个共有峰，其精密度、重复性、稳定性试验中各共有峰的相对保留时间的 rsd 均小于2%，相对峰面积的 rsd 均小于5%。模拟片相似度较好,银翘解毒片样品图谱存在较大差异。在已建立特征图谱基础上，提取3个特征色谱峰及1个组峰作为甘草、连翘、金银花、牛蒡子4味药材归属的样品特征峰。结论建立银翘解毒片 HPlc 特征图谱分析方法，为进一步控制银翘解毒片样品质量提供研究基础。
목적：건립은교해독편특정도보적측정방법，정체평개은교해독편적질량。방법채용색보주：Kromasil 100-5 c18(4.6 mm ×250 mm)；검측기：dad 검측기；검측파장：279nm；주온：30℃；갑순-0.2%린산위류동상，1ml·min-1적류속제도세탈。결과모의은교해독편제특정도보유12개공유봉，기정밀도、중복성、은정성시험중각공유봉적상대보류시간적 rsd 균소우2%，상대봉면적적 rsd 균소우5%。모의편상사도교호,은교해독편양품도보존재교대차이。재이건립특정도보기출상，제취3개특정색보봉급1개조봉작위감초、련교、금은화、우방자4미약재귀속적양품특정봉。결론건립은교해독편 HPlc 특정도보분석방법，위진일보공제은교해독편양품질량제공연구기출。
Objective to establish method for determination of the fructus forsythiae detoxify piece of feature maps, the overall assessment of the quality of fructus forsythiae detoxify piece. Methods the chromatographic column: Kromasil 100-5 c18 (4.6 mm * 250 mm); detector: dad detector; : detection wavelength of 279 nm; column temperature: 30 ℃ ; Methanol- 0.2% phosphoric acid as mobile phase, 1 ml, min - 1 the velocity gradient elution. Results the fructus forsythiae jiedu tablet feature maps are 12 common peak, its precision, repeatability and stability test of each common peak in the relative retention time of the RSD are less than 2%, the RSD of relative peak area were less than 5%. Simulated better similarity, fructus forsythiae jiedu tablet sample map there is a big difference. on the basis of feature maps has been established, three feature extracting and 1 set of chromatographic peak peak as liquorice, forsythia, flos lonicerae, fructus arctii 4 samples of medicinal materials of characteristic peak. Conclusion to establish fructus forsythiae jiedu tablets HPlc feature maps analysis method, in order to further control of fructus forsythiae jiedu tablet sample quality provides research foundation.