北华大学学报:自然科学版
北華大學學報:自然科學版
북화대학학보:자연과학판
Journal of Beihua University(Natural Science)
2012年
1期
53-57
,共5页
沈维高%王跃华%缪春明%刘艳波%孙雪梅%任建新%何欣
瀋維高%王躍華%繆春明%劉豔波%孫雪梅%任建新%何訢
침유고%왕약화%무춘명%류염파%손설매%임건신%하흔
羟基磷灰石%纳米粒子%Stat3%胶质瘤
羥基燐灰石%納米粒子%Stat3%膠質瘤
간기린회석%납미입자%Stat3%효질류
hydroxyapatite%nanoparticle%Stat3%glioma
目的探讨羟基磷灰石纳米粒子运载Star3 shRNA对鼠胶质瘤C6细胞的促凋亡作用,并探讨相关机制.方法羟基磷灰石纳米粒子包被的Stat3 shRNA转染到c6胶质瘤细胞内,MTT法检测细胞增殖情况,应用流式细胞术及吖啶橙染色观察细胞周期分布及凋亡情况,TUNEL染色观察细胞的凋亡及增殖情况.结果羟基磷灰石纳米粒子运载Star3 shRNA转染C6细胞后,呈时间依赖性抑制C6细胞生长和增殖.通过流式细胞术检测证实:该质粒可使细胞阻滞在细胞周期的GO/G1期,细胞凋亡率明显增加(P<0.05),与对照组比较差异有统计学意义;吖啶橙及TUNEL染色发现其明显促进细胞凋亡(P<0.05),与对照组比较差异有统计学意义;结论羟基磷灰石纳米粒子运载Stat3 shRNA体外可明显抑制胶质瘤C6细胞增殖,并促进其凋亡,是治疗胶质瘤较理想的方法.
目的探討羥基燐灰石納米粒子運載Star3 shRNA對鼠膠質瘤C6細胞的促凋亡作用,併探討相關機製.方法羥基燐灰石納米粒子包被的Stat3 shRNA轉染到c6膠質瘤細胞內,MTT法檢測細胞增殖情況,應用流式細胞術及吖啶橙染色觀察細胞週期分佈及凋亡情況,TUNEL染色觀察細胞的凋亡及增殖情況.結果羥基燐灰石納米粒子運載Star3 shRNA轉染C6細胞後,呈時間依賴性抑製C6細胞生長和增殖.通過流式細胞術檢測證實:該質粒可使細胞阻滯在細胞週期的GO/G1期,細胞凋亡率明顯增加(P<0.05),與對照組比較差異有統計學意義;吖啶橙及TUNEL染色髮現其明顯促進細胞凋亡(P<0.05),與對照組比較差異有統計學意義;結論羥基燐灰石納米粒子運載Stat3 shRNA體外可明顯抑製膠質瘤C6細胞增殖,併促進其凋亡,是治療膠質瘤較理想的方法.
목적탐토간기린회석납미입자운재Star3 shRNA대서효질류C6세포적촉조망작용,병탐토상관궤제.방법간기린회석납미입자포피적Stat3 shRNA전염도c6효질류세포내,MTT법검측세포증식정황,응용류식세포술급아정등염색관찰세포주기분포급조망정황,TUNEL염색관찰세포적조망급증식정황.결과간기린회석납미입자운재Star3 shRNA전염C6세포후,정시간의뢰성억제C6세포생장화증식.통과류식세포술검측증실:해질립가사세포조체재세포주기적GO/G1기,세포조망솔명현증가(P<0.05),여대조조비교차이유통계학의의;아정등급TUNEL염색발현기명현촉진세포조망(P<0.05),여대조조비교차이유통계학의의;결론간기린회석납미입자운재Stat3 shRNA체외가명현억제효질류C6세포증식,병촉진기조망,시치료효질류교이상적방법.
Objective This study is aimed to determine the pro-apoptotic effect of Stat3 shRNA delivered by hydroxyapatite nanoparticles on glioblastoma C6 cells and explore the related mechanisms. Method Stat3 shRNA was delivered into rat C6 glioma cells by hydroxyapatite nanoparticles. Cell growth was determined by MTF assay,and cell cycle distribution and apoptosis by flow cytometric and ethidium bromide/acridine orange staining assays. Apoptosis and proliferation of the C6 cells were determined by TUNEL assay. Results After Stat3 shRNA was transfected into C6 glioma cells through the hydroxyapatite nanoparticle delivery approach, the growth and proliferation of the ceils were considerably inhibited in a time-dependent way, and the flow cytometry verified that the cells were arrested in G0/G1 stage of the cell cycle and the apoptosis was significantly increased comparing with those of the control group ( P 〈 0.05 ). Ethidium bromide/acridine orange staining and TUNEL staining demonstrated that it also promoted C6 cells apoptosis obviously comparing with control group (P〈0.05). Conclusion Stat3 shRNA delivered by hydroxyapatite nanoparticles in vitro could significantly inhibit the growth of rat C6 glioma cells and promote their apoptosis,which may be an ideal method for the treatment of glioma.