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LVSE DASHIJIU
2012年
5期
1-2
,共2页
新几内亚凤仙%增殖%试管内开花
新幾內亞鳳仙%增殖%試管內開花
신궤내아봉선%증식%시관내개화
Impaliens hawkeri%proliferations flowering in vitro
以新几内亚凤仙花种子无菌萌发的试管苗为材料,探讨了激素对芽增殖、生根以及试管内开花的影响。结果表明:芽增殖最适培养基为Ms+0.5mg/L6-BA+0.5mg/LNAA,35d顸芽增殖倍数为3.0,侧芽增殖倍数为5.3,生根率同步达100%;试管内诱导开花的最适培养基为Ms+2.0mg/L6-BA-+-0.2mg/LNAA,其开花率达90%,平均每颗苗开花数为2.2朵,花期长达60d。
以新幾內亞鳳仙花種子無菌萌髮的試管苗為材料,探討瞭激素對芽增殖、生根以及試管內開花的影響。結果錶明:芽增殖最適培養基為Ms+0.5mg/L6-BA+0.5mg/LNAA,35d頇芽增殖倍數為3.0,側芽增殖倍數為5.3,生根率同步達100%;試管內誘導開花的最適培養基為Ms+2.0mg/L6-BA-+-0.2mg/LNAA,其開花率達90%,平均每顆苗開花數為2.2朵,花期長達60d。
이신궤내아봉선화충자무균맹발적시관묘위재료,탐토료격소대아증식、생근이급시관내개화적영향。결과표명:아증식최괄배양기위Ms+0.5mg/L6-BA+0.5mg/LNAA,35d한아증식배수위3.0,측아증식배수위5.3,생근솔동보체100%;시관내유도개화적최괄배양기위Ms+2.0mg/L6-BA-+-0.2mg/LNAA,기개화솔체90%,평균매과묘개화수위2.2타,화기장체60d。
Embryo bud of Impatiens hawkeri was cultured on hormone--free MS medium for proliferation and flowering in vitro. The results showed that the optimum medium of shoots proliferation was MS + 0.5mg/l. 6-BA -I-0.5mg/L NAA;the proliferation coefficient of terminal bud was 3.0,bul lateral budding was 5.3 for thirty--five day. In addition, the percentage of rooting was 100% synchronously. The optimum medium of flowering in vitro was MS+ 2.0mg/I. 6--BA +0.2mg/I. NAA. The percentage of flowering reached 90% and the average flowers per tissue culture seedling was 2.2 as well as flowering for sixty days.