分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2013年
3期
431-436
,共6页
林佳%尹晓雯%郑蕊%章英才%徐青
林佳%尹曉雯%鄭蕊%章英纔%徐青
림가%윤효문%정예%장영재%서청
枸杞%花药线粒体%分离纯化%方法
枸杞%花藥線粒體%分離純化%方法
구기%화약선립체%분리순화%방법
Lycium barbarum L.%Anther mitochondria%Extracting and purifying%Method
为了获得高质量的枸杞花药线粒体，本文介绍了一种差速离心分离纯化枸杞花药线粒体的方法。通过Jannus Green B显色反应、线粒体跳动特性以及电镜观察鉴定，结果表明采用本差速离心法提取的线粒体纯度高、活性强、结构完整。进一步通过琼脂糖电泳、光密度值及核特异引物PCR检测表明，由该线粒体裂解的mtDNA不仅纯度高、质量好，而且得率高。本差速离心法具有简捷、高效和经济等优点。用该方法获得的线粒体完全能够满足线粒体基因组、蛋白质组等相关分子生物学研究要求。
為瞭穫得高質量的枸杞花藥線粒體，本文介紹瞭一種差速離心分離純化枸杞花藥線粒體的方法。通過Jannus Green B顯色反應、線粒體跳動特性以及電鏡觀察鑒定，結果錶明採用本差速離心法提取的線粒體純度高、活性彊、結構完整。進一步通過瓊脂糖電泳、光密度值及覈特異引物PCR檢測錶明，由該線粒體裂解的mtDNA不僅純度高、質量好，而且得率高。本差速離心法具有簡捷、高效和經濟等優點。用該方法穫得的線粒體完全能夠滿足線粒體基因組、蛋白質組等相關分子生物學研究要求。
위료획득고질량적구기화약선립체，본문개소료일충차속리심분리순화구기화약선립체적방법。통과Jannus Green B현색반응、선립체도동특성이급전경관찰감정，결과표명채용본차속리심법제취적선립체순도고、활성강、결구완정。진일보통과경지당전영、광밀도치급핵특이인물PCR검측표명，유해선립체렬해적mtDNA불부순도고、질량호，이차득솔고。본차속리심법구유간첩、고효화경제등우점。용해방법획득적선립체완전능구만족선립체기인조、단백질조등상관분자생물학연구요구。
In order to obtain high quality anther mitochondria, this paper introduces a method of extracting and purifying anther mitochondria in Lycium barbarum L. by differential centrifugation. The results showed that mito-chondria of using the method extracting are pure, strong activity and complete structure by the Jannus Green B staining, beating characteristics of mitochondria and identification of electron microscopy. And furthermore results showed that mtDNA form the mitochondria not only are high purity and good quality, but also high yield through the detecting of the agarose gel electrophoresis, optical density and nuclear specific primer PCR. It indicates that this method has easy, quick, efficient and economic advantages. And the mitochondria of using the method extracting can completely meet the research requirements of the mitochondrial genome, proteome and other related molecular biology experiment.
