CAJ | 학술논문

目的：通过体外诱导探索肿瘤相关巨噬细胞（TAM）及肿瘤坏死因子-α（TNF-α）对肿瘤细胞增殖和迁移的影响；在此基础上研究羧胺三唑（CAI）是否可以通过影响TAM及其来源的TNF-α间接抑制肿瘤细胞的增殖和迁移。方法建立腹腔巨噬细胞或RAW264.7细胞与Lewis 肺癌细胞（LLC）的共培养体系，研究巨噬细胞对 LLC 细胞增殖和迁移的影响；观察TNF-α或其中和抗体对LLC增殖或迁移的影响；向共培养体系中加入CAI和（或）地塞米松（DEX），观察药物对巨噬细胞诱导的LLC增殖的影响；用CAI和（或）DEX预先处理RAW264.7细胞，然后检测不同药物处理组RAW264.7细胞诱导LLC细胞迁移和TNF-α表达的差异。结果与腹腔巨噬细胞共培养的 LLC细胞比单独培养的LLC细胞的增殖显著增加，在最为明显的一组中，前者比后者的增殖增加（422.5±77.7）%；与 RAW264.7细胞共培养的 LLC 细胞比单独培养的 LLC 细胞迁移增加（98.8±6.2）%。在这两个过程中，TNF-α均发挥重要作用，0.1 ng/ml TNF-α增加LLC细胞增殖的作用显著，0.1μg/ml TNF-α中和抗体即可显著抑制巨噬细胞对LLC细胞的迁移诱导作用；CAI预处理的RAW264.7细胞促进LLC细胞迁移的能力减弱，其中TNF-α表达相比仅用LLC条件培养基诱导的对照组显著降低（CAI预处理组与对照组TNF-α的相对表达量为0.66±0.03 vs.1.00±0.05，P＜0.01）。结论巨噬细胞和TNF-α对LLC细胞的增殖和迁移有显著影响；CAI可以通过下调肿瘤条件诱导的巨噬细胞中的TNF-α水平间接抑制肿瘤细胞的迁移。
목적：통과체외유도탐색종류상관거서세포（TAM）급종류배사인자-α（TNF-α）대종류세포증식화천이적영향；재차기출상연구최알삼서（CAI）시부가이통과영향TAM급기래원적TNF-α간접억제종류세포적증식화천이。방법건립복강거서세포혹RAW264.7세포여Lewis 폐암세포（LLC）적공배양체계，연구거서세포대 LLC 세포증식화천이적영향；관찰TNF-α혹기중화항체대LLC증식혹천이적영향；향공배양체계중가입CAI화（혹）지새미송（DEX），관찰약물대거서세포유도적LLC증식적영향；용CAI화（혹）DEX예선처리RAW264.7세포，연후검측불동약물처리조RAW264.7세포유도LLC세포천이화TNF-α표체적차이。결과여복강거서세포공배양적 LLC세포비단독배양적LLC세포적증식현저증가，재최위명현적일조중，전자비후자적증식증가（422.5±77.7）%；여 RAW264.7세포공배양적 LLC 세포비단독배양적 LLC 세포천이증가（98.8±6.2）%。재저량개과정중，TNF-α균발휘중요작용，0.1 ng/ml TNF-α증가LLC세포증식적작용현저，0.1μg/ml TNF-α중화항체즉가현저억제거서세포대LLC세포적천이유도작용；CAI예처리적RAW264.7세포촉진LLC세포천이적능력감약，기중TNF-α표체상비부용LLC조건배양기유도적대조조현저강저（CAI예처리조여대조조TNF-α적상대표체량위0.66±0.03 vs.1.00±0.05，P＜0.01）。결론거서세포화TNF-α대LLC세포적증식화천이유현저영향；CAI가이통과하조종류조건유도적거서세포중적TNF-α수평간접억제종류세포적천이。
Objective To investigate the effects of tumor associated macrophages(TAM) and tumor necrosis factor-α(TNF-α) on tumor cell proliferation and migration in the induction systems established in vitro. To investigate whether carboxyamidotriazole(CAI) can inhibit the tumor cell proliferation and migration indirectly by down-regulating the TNF-αexpression in TAM. Methods The peritoneal macrophages or RAW264.7 macrophages were co-cultured with the Lewis lung carcinoma (LLC) cells, and the effects of the macrophages on LLC proliferation and migration were assayed with CCK-8 and the crystal violet, respectively. TNF-α or its neutralizing antibody was added to stimulate or block LLC proliferation or migration. The effects of CAI and/or dexamethasone(DEX) on LLC proliferation induced by peritoneal macrophages were observed after adding them to the co-culture system. RAW264.7 was pre-treated with CAI and/or DEX, and the ability to induce LLC migration and the TNF-αexpression in RAW264.7 with different treatments were investigated. Results The proliferation and migration of LLC cells in the co-culture systems were significantly enhanced compared with the cells cultured alone. The proliferation and migration of the co-cultured LLC cells could be increased by (422.5±77.7)%and (98.8± 6.2)%, respectively. The proliferation could be significantly stimulated by low level of TNF-α(0.1 ng/ml). LLC migration induced by macrophages could be greatly inhibited by the TNF-α neutralizing antibody. The ability of RAW264.7 to induce LLC migration was impaired and the TNF-αexpression in RAW264.7 was down-regulated after CAI treatment (relative expression of TNF-α in CAI pre-treatment group and control group were 0.66±0.03 vs. 1.00±0.05, P<0.01). Conclusion Macrophages and TNF-α have significant effects on LLC proliferation and migration in the co-culture systems in vitro. CAI can indirectly inhibit the tumor cell migration by down-regulating the TNF-αexpression in TAMs.