CAJ | 학술논문

　　以防风茎段为外植体，建立了组织培养再生体系，对防风试管苗玻璃化现象进行了研究。结果表明，与正常苗相比，玻璃化苗形态异常，组织含水量升高，叶绿素含量显著降低，酸性过氧化物同工酶活性减弱。6-BA浓度超过2.0 mg/L、光照低于2000 lx、培养瓶内湿度大都极易导致防风试管苗的玻璃化，减少愈伤继代次数，增加培养基内琼脂和蔗糖浓度，可以降低玻璃化率。轻中度的玻璃化苗通过改变培养环境可以恢复正常。优化的防风再生体系为：以嫩茎段为外植体，继代3次左右的愈伤组织诱导出芽，芽继代增殖时，6-BA浓度采用1.0 mg/L和0.5 mg/L交替使用，培养光照3000~4000 lx。
　　이방풍경단위외식체，건립료조직배양재생체계，대방풍시관묘파리화현상진행료연구。결과표명，여정상묘상비，파리화묘형태이상，조직함수량승고，협록소함량현저강저，산성과양화물동공매활성감약。6-BA농도초과2.0 mg/L、광조저우2000 lx、배양병내습도대도겁역도치방풍시관묘적파리화，감소유상계대차수，증가배양기내경지화자당농도，가이강저파리화솔。경중도적파리화묘통과개변배양배경가이회복정상。우화적방풍재생체계위：이눈경단위외식체，계대3차좌우적유상조직유도출아，아계대증식시，6-BA농도채용1.0 mg/L화0.5 mg/L교체사용，배양광조3000~4000 lx。
Stems of Saposhnikovia divaricata were cultured in vitro to establish the regeneration system, and the vitrification of plantlets was also researched. The results indicated that the appearance was different with vitreous plantlets, vitreous plantlets had more water and fewer chlorophall. The acidic bands' activity of peroxidase (POD) isozymes was decreased. 6-BA concentration being more than 2.0 mg/L、light intensity being lower than 2 000 lx and high humidity easily induced the vitreous plantlets and the percentage of vitrification could be reduced by cutting down callus' ages and increasing sucrose and agar concentration. Mild and moderate vitrification could be reversed by changing cultural condition. The optimized regeneration system was: Tender stems were used as explants;using callus about 3 ages for buds' differentiation, and 6-BA concentration was alternated 1.0 mg/L with 0.5 mg/L on subculture;Choosing light intensity on 3 000~4 000 lx.