CAJ | 학술논문

　　百合杂交育种工作中，只有确定杂种的真实性，才能确定杂交工作是否成功。利用ISSR-PCR分子标记方法对5个亚洲百合系内杂种进行鉴定，建立了百合ISSR最佳扩增反应体系，从15个ISSR引物中筛选出了2个多态性引物，共扩增得到29条DNA条带，其中多态性条带24条，占总条带数的82.76%。结果表明，5个杂种多数条带和亲本共有，出现双亲相加性带型，或双亲各自特有条带，部分杂种出现双亲均没有的条带从这些特异的带型，我们可初步鉴定杂种的真实性。实践证明，ISSR分子标记，是百合杂交育种中杂种检测的快速简便手段。
　　백합잡교육충공작중，지유학정잡충적진실성，재능학정잡교공작시부성공。이용ISSR-PCR분자표기방법대5개아주백합계내잡충진행감정，건립료백합ISSR최가확증반응체계，종15개ISSR인물중사선출료2개다태성인물，공확증득도29조DNA조대，기중다태성조대24조，점총조대수적82.76%。결과표명，5개잡충다수조대화친본공유，출현쌍친상가성대형，혹쌍친각자특유조대，부분잡충출현쌍친균몰유적조대종저사특이적대형，아문가초보감정잡충적진실성。실천증명，ISSR분자표기，시백합잡교육충중잡충검측적쾌속간편수단。
Only we confirmed the authenticity of the hybrid, can we identify whether the results of Lily cross-breeding are successful, which provides the basis for the following breeding. Genomic DNAs were extracted from young leaves of 5hybrids and their parents. PCR conditions suitable for ISSR (inter-simple sequence repeat) molecular marker were studied. 2 polymorphism primers, (CT)7AAA and (CT)7AGT, selected from 15 ISSR primers were used for parentage identification. 24 polymorphic bands was obtained among a total of 29 DNA bands (82.76%), which was useful in matching parents and hybrids. The five hybrids share the majority bands with parents (father or mother) while some have unique bands showing the authenticity of the hybrids. The results indicate that parentage identification by ISSR is effective in cross breeding hybrid of Asian lily.