CAJ | 학술논문

　　本研究采用AFLP分子标记技术，对采集于四川省和云南省的56株滇杨(Populus yunnanensis Dode)优树基因组遗传变异、54个滇杨优树无性系3a苗木落叶期侧芽的基因表达谱进行了分析。DNA-AFLP分析结果表明，筛选出的8对AFLP引物组合共扩增出508条带，平均多态性条带比率为59.25%，观测等位基因数为1.5925，有效等位基因数为1.1581，Nei's基因多样性指数为0.1129，Shannon's信息指数为0.1914；除有效等位基因数相等外，四川滇杨优树群体的4项遗传多样性指标均略高于云南滇杨优树群体，但2个优树群体之间存在着较高的基因流(Nm=21.64)。 cDNA-AFLP分析结果显示，筛选出的8对AFLP引物组合共扩增出526条带，其中平均差异性条带百分率为82.70%，观测等位基因数和有效等位基因数分别为1.8270和1.2417，Nei's基因多样性指数为0.1675，Shannon's信息指数为0.2809；四川滇杨优树群体的基因表达差异性指标均略高于云南滇杨优树群体，且二者之间的基因流值较大(Nm=27.00)。分子方差分析(AMOVA)结果表明，滇杨2个优树群体在DNA水平和cDNA水平的遗传差异均极显著。
　　본연구채용AFLP분자표기기술，대채집우사천성화운남성적56주전양(Populus yunnanensis Dode)우수기인조유전변이、54개전양우수무성계3a묘목락협기측아적기인표체보진행료분석。DNA-AFLP분석결과표명，사선출적8대AFLP인물조합공확증출508조대，평균다태성조대비솔위59.25%，관측등위기인수위1.5925，유효등위기인수위1.1581，Nei's기인다양성지수위0.1129，Shannon's신식지수위0.1914；제유효등위기인수상등외，사천전양우수군체적4항유전다양성지표균략고우운남전양우수군체，단2개우수군체지간존재착교고적기인류(Nm=21.64)。 cDNA-AFLP분석결과현시，사선출적8대AFLP인물조합공확증출526조대，기중평균차이성조대백분솔위82.70%，관측등위기인수화유효등위기인수분별위1.8270화1.2417，Nei's기인다양성지수위0.1675，Shannon's신식지수위0.2809；사천전양우수군체적기인표체차이성지표균략고우운남전양우수군체，차이자지간적기인류치교대(Nm=27.00)。분자방차분석(AMOVA)결과표명，전양2개우수군체재DNA수평화cDNA수평적유전차이균겁현저。
The genomes genetic variation of 56 plus trees and the gene transcription profiling of lateral buds in defoliation period of 54 three-year old seedlings of plus tree clones in Populus yunnanensis, collected from Sichuan Province and Yunnan Province, were detected by AFLP markers. The results by DNA-AFLP showed that 508 bands were amplified by 8 selected prime combinations, the ratio of polymorphic bands on average was 59.25%, the number of alleles was 1.592 5, the effective number of alleles was 1.158 1, the Nei's gene diversity index was 0.112 9, and Shannon's information index was 0.191 4. The values of genetic diversity index of Sichuan popula-tion were slightly higher than the values of Yunnan population except the effective number of alleles, which was equal between two populations. However, higher gene flow was existed between the two populations (Nm=21.64). In cDNA-AFLP analysis, a total of 526 informative AFLP markers were generated by 8 selected primer combina-tions, which showed a 82.70% level of difference on average. The number of alleles, the effective number of alleles, the Nei's gene diversity index and Shannon's information index was 1.827 0, 1.241 7, 0.167 5 and 0.280 9,respectively. All of the values of gene differential expression index of Sichuan population were slightly higher than the values of Yunnan population, and it was also that a high gene flow value (Nm=27.00) existed between the two populations. Analysis of molecular variance (AMOVA) suggested that genetic variance between the two populations were significantly different at DNA and cDNA level.