CAJ | 학술논문

　　建立了牛奶中11种光引发剂残留的定性和定量测定分析方法。样品经Carrez试剂沉淀蛋白质后，使用乙腈提取，HLB固相萃取柱净化，乙腈洗脱，洗脱液用氮气吹干后用50％乙腈水溶液定容，采用液相色谱-串联质谱进行测定。分析物采用电喷雾电离，正离子扫描，多反应监测（MRM）模式检测，其中7种光引发剂使用内标法定量，4种光引发剂使用外标法定量。11种光引发剂在5．00～200μg/L浓度范围内线性关系良好，相关系数均大于0．990。11种光引发剂的测定低限均为1．0μg/kg。在添加浓度1．00～100μg/kg范围内，加标回收率为74．3％～112％，相对标准偏差为0．57％～16．3％。本方法简便、有效、灵敏，适用于牛奶中光引发剂多残留的定量测定和确证。
　　건립료우내중11충광인발제잔류적정성화정량측정분석방법。양품경Carrez시제침정단백질후，사용을정제취，HLB고상췌취주정화，을정세탈，세탈액용담기취간후용50％을정수용액정용，채용액상색보-천련질보진행측정。분석물채용전분무전리，정리자소묘，다반응감측（MRM）모식검측，기중7충광인발제사용내표법정량，4충광인발제사용외표법정량。11충광인발제재5．00～200μg/L농도범위내선성관계량호，상관계수균대우0．990。11충광인발제적측정저한균위1．0μg/kg。재첨가농도1．00～100μg/kg범위내，가표회수솔위74．3％～112％，상대표준편차위0．57％～16．3％。본방법간편、유효、령민，괄용우우내중광인발제다잔류적정량측정화학증。
A method for the qualitative and quantitative determination of 11 photoinitiators residues in milk by liquid chroma-tography-tandem mass spectrometry (LC-TMS) was developed.The samples were extracted with acetonitrile after being precipi-tated by Carrez reagent, and then cleaned by HLB solid phase extraction cartridge .The analytes were eluted by acetonitrile , and then reconstituted by 50%acetonitrile aqueous solution after being dried by nitrogen gas .LC-TMS was used for the determination. The analytes were determined by the mode of electro -spray ionization (ESI), positive ion scan, and multiple reaction monitoring (MRM), and internal standard method for the quantification of 7 photoinitiators and external standard method for the quantification of other 4 photoinitiators were used.Eleven photoinitiators within the concentration range of 5.00~200μg/L showed good linear re-lationship with the correlation coefficient of more than 0.990.All the lowest limits of the quantitative determination were 1.00 μg/kg.The recoveries of 11 photoinitiators were between 74.3%and 112% when the adding concentrations were 1.00~100 μg/kg, and the relative standard deviations varied from 0.57%to 16.3%.This method was simple, effective and sensitive, and suitable for the quantitative determination and confirmation of photoinitiator residues in milk .