CAJ | 학술논문

　　以阿蒂擎天凤梨乙烯处理和不处理的植株为材料建立的抑制性差减杂交文库中，筛选到的一个被乙烯诱导并与已知植物谷胱甘肽-S-转移酶(GST)同源的cDNA片段，通过RACE技术得到该基因的全长cDNA序列。序列分析表明，该基因可能属于thioredoxin-like superfamily和GST C_family superfamily两个超家族中的成员。利用半定量RT-PCR检测该基因的表达量在乙烯处理后先会在1 h显著升高，然后随时间逐步降低，说明乙烯作为一种逆境胁迫相关激素，可在短时间内诱导凤梨中GST的表达。推测其在受到外源乙烯信号诱导后，一方面可能参与了植物体内的解毒作用，另一方面可能参与了花青素的合成调控和运输。本实验中的GST作为观赏凤梨中一个新发现的GSTs家族成员，对于研究热带花卉中GSTs家族的特点和提高其抗逆性和品质具有重要的理论意义和实用价值。
　　이아체경천봉리을희처리화불처리적식주위재료건립적억제성차감잡교문고중，사선도적일개피을희유도병여이지식물곡광감태-S-전이매(GST)동원적cDNA편단，통과RACE기술득도해기인적전장cDNA서렬。서렬분석표명，해기인가능속우thioredoxin-like superfamily화GST C_family superfamily량개초가족중적성원。이용반정량RT-PCR검측해기인적표체량재을희처리후선회재1 h현저승고，연후수시간축보강저，설명을희작위일충역경협박상관격소，가재단시간내유도봉리중GST적표체。추측기재수도외원을희신호유도후，일방면가능삼여료식물체내적해독작용，령일방면가능삼여료화청소적합성조공화운수。본실험중적GST작위관상봉리중일개신발현적GSTs가족성원，대우연구열대화훼중GSTs가족적특점화제고기항역성화품질구유중요적이론의의화실용개치。
An ethylene inducible cDNA fragment being homology with the known glutathione-s-transferase (GST) in plants was screened in the suppression subtractive hybridization (SSH) library constructed with the materials of Guzmania wittmackii‘Attila’ plant treated and untreated by ethylene. Rapid Amplification of cDNA Ends (RACE) was used to obtain the full length cDNA. Sequence analysis suggested that the gene should belong to thioredoxin-like superfamily and GST C_family superfamily. Semi-quantitative RT-PCR results exhibited that the expre ssion of this gene significantly increased with the treatment of ethylene in one hour, and then decreased gradually with time, which suggested that the ethylene as an adversity stress-related hormone could induce GST gene expression in a short time. We hypothesized that GST, on the one hand, might be involved in detoxification in vivo, on the other hand, might be involved in regulation and transport of anthocyanin after ethylene induction. In our research, this GST as one new member of GSTs family in bromeliads, has the essential theoretical significance and applied value to study GSTs family characteristics and improve stress resistance and quality modification in tropical flowers.