分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2013年
3期
351-357
,共7页
马铃薯%玻璃化法%超低温保存%DNA甲基化%甲基化敏感扩增多态性%遗传变异
馬鈴藷%玻璃化法%超低溫保存%DNA甲基化%甲基化敏感擴增多態性%遺傳變異
마령서%파리화법%초저온보존%DNA갑기화%갑기화민감확증다태성%유전변이
Potato%Cryopreservation%Vitrification%DNA methylation%Methylation-sensitive amplification
对青薯9号、大西洋、小白花和E1074种基因型的马铃薯茎尖进行玻璃化法超低温保存，并运用甲基化敏感扩增多态性(MSAP)技术分析4种材料超低温保存前后DNA甲基化的的变异情况。结果表明：经玻璃化法超低温保存，四个品种的成活率分别为86.93%、78.03%、71.57%和65.82%。成活率和再生率因基因型而异，且不同基因型之间的差异显著。用MSAP技术分析超低温保存前后植株甲基化的结果显示：用16对引物组合对4个品种进行扩增，平均扩增出493条带；与对照相比，4个品种基因组的CCGG序列中有8.4%~14.3%DNA发生甲基化变化。经超低温保存后，去甲基化和甲基化都有发生，并以去甲基化变化为主要趋势。本文运用MSAP技术对马铃薯超低温保存前后植株进行胞嘧啶甲基化分析表明，使用MSAP检测超低温保存后再生植株DNA甲基化遗传变异非常有效。
對青藷9號、大西洋、小白花和E1074種基因型的馬鈴藷莖尖進行玻璃化法超低溫保存，併運用甲基化敏感擴增多態性(MSAP)技術分析4種材料超低溫保存前後DNA甲基化的的變異情況。結果錶明：經玻璃化法超低溫保存，四箇品種的成活率分彆為86.93%、78.03%、71.57%和65.82%。成活率和再生率因基因型而異，且不同基因型之間的差異顯著。用MSAP技術分析超低溫保存前後植株甲基化的結果顯示：用16對引物組閤對4箇品種進行擴增，平均擴增齣493條帶；與對照相比，4箇品種基因組的CCGG序列中有8.4%~14.3%DNA髮生甲基化變化。經超低溫保存後，去甲基化和甲基化都有髮生，併以去甲基化變化為主要趨勢。本文運用MSAP技術對馬鈴藷超低溫保存前後植株進行胞嘧啶甲基化分析錶明，使用MSAP檢測超低溫保存後再生植株DNA甲基化遺傳變異非常有效。
대청서9호、대서양、소백화화E1074충기인형적마령서경첨진행파리화법초저온보존，병운용갑기화민감확증다태성(MSAP)기술분석4충재료초저온보존전후DNA갑기화적적변이정황。결과표명：경파리화법초저온보존，사개품충적성활솔분별위86.93%、78.03%、71.57%화65.82%。성활솔화재생솔인기인형이이，차불동기인형지간적차이현저。용MSAP기술분석초저온보존전후식주갑기화적결과현시：용16대인물조합대4개품충진행확증，평균확증출493조대；여대조상비，4개품충기인조적CCGG서렬중유8.4%~14.3%DNA발생갑기화변화。경초저온보존후，거갑기화화갑기화도유발생，병이거갑기화변화위주요추세。본문운용MSAP기술대마령서초저온보존전후식주진행포밀정갑기화분석표명，사용MSAP검측초저온보존후재생식주DNA갑기화유전변이비상유효。
Cryopreservation by using vitrification approach was carried out to preserve the shoot tips of four genotype potatoes, Qingshu 9 hao, Atlantic, Xiaobaihua and E107, and the genetic variation patterns of DNA methylation in four potato genotypes were assessed by using the technique of methylation-sensitive amplified polymorphism (MSAP). The results showed that the survival rates of four genotypes potato were 86.93%, 78.03%, 71.57% and 65.82%, respectively. The survival and regeneration rates varied with genotypes of potatoes, as well as significant difference between different genotypes. MSAP analysis on the plant methylation indicated that average 493 fragments each genotype were amplified using 16 pairs of selective primers, Compared to the control, DNA methylation events were detected in the percentages from 8.4% to 14.3% in the CCGG sequence of potato genomes. Demethylation and de novo methylation were produced after cryopreservation, and demethylation was the main trend of methylation changes. This paper analyse cytosine methylation in potato plant using MSAP, indicating MSAP technique is efficient for detection of genetic variation in regeneration plant after cryopreservation.
