CAJ | 학술논문

　　本研究构建了含有耐草甘膦基因G2-epsps和R79-epsps的双价植物表达载体pCMG2R79-EPSPS，以优良玉米自交系78599和综31的幼胚为受体材料，采用农杆菌介导法将耐草甘膦双价基因G2R79-epsps转入玉米幼胚细胞，以期获得耐草甘膦性更高的转基因玉米植株。目前已获得转基因再生玉米植株32株，经特异PCR检测表明其中7株植株转入了目标基因，阳性率达到21.9%。对得到的阳性植株的T1、T2代进行了跟踪检测，经田间喷施除草剂试验结果表明，获得了可耐草甘膦6‰的玉米材料，即生产上草甘膦田间使用浓度3倍水平的材料，为抗除草剂玉米新品种选育提供了较好的基础材料。
　　본연구구건료함유내초감련기인G2-epsps화R79-epsps적쌍개식물표체재체pCMG2R79-EPSPS，이우량옥미자교계78599화종31적유배위수체재료，채용농간균개도법장내초감련쌍개기인G2R79-epsps전입옥미유배세포，이기획득내초감련성경고적전기인옥미식주。목전이획득전기인재생옥미식주32주，경특이PCR검측표명기중7주식주전입료목표기인，양성솔체도21.9%。대득도적양성식주적T1、T2대진행료근종검측，경전간분시제초제시험결과표명，획득료가내초감련6‰적옥미재료，즉생산상초감련전간사용농도3배수평적재료，위항제초제옥미신품충선육제공료교호적기출재료。
A bivalent plant expression vector pCMG2R79-EPSPS expressing the glyphosatetolerant G2-epsps gene and R79-epsps gene was constructed and transformed into the immature embryos of the elite maize lines 78599 and Zong31, for gaining more higher glyphosate-tolerance of transgenic maize plants. To this day, 32 regeneration plants was acquired and the specific PCR detection showed that 7 plants of them were transferred with the target gene. The positive rate was 21.9%. Further analysis was performed on the T1 and T2 generation and it was.obtained 6‰concentration glyphosate-resistant plants, that was 3 times concentration levels than the glyphosate commer-cial using did, by spraying herbicide glyphosate in field. It would provide a kind of better parent materials for breeding the new resistant herbicide glyphosate maize varieties.