CAJ | 학술논문

　　根据MaizeDGB数据库提供的CIPK序列(GRMZM2G011919)，克隆得到一个全长为1908 bp的ZmCIPK42基因，其开放阅读框(ORF)为1323 bp，编码一个440氨基酸残基的多肽。 ZmCIPK42具有CIPK基因家族典型的激酶结构域和NAF调控结构域，其氨基酸序列与玉米CIPK15(NP_001108478)、水稻CIP-K14(Q2QYM3.1)、小麦CIPK14(AFR90220.1)和大麦CIPK14(AEZ51505)等高等植物的CIPK氨基酸序列的同源性分别为74%、73%、73%和70%。与拟南芥CIPK家族系统进化分析表明ZmCIPK42与拟南芥CIPK2和CIPK10同源关系较近。荧光定量PCR分析发现ZmCIPK42基因在盐胁迫和热处理后表达量显著升高，脱水处理后表达水平也有所提高，ABA和冷处理后表达量变化不明显。
　　근거MaizeDGB수거고제공적CIPK서렬(GRMZM2G011919)，극륭득도일개전장위1908 bp적ZmCIPK42기인，기개방열독광(ORF)위1323 bp，편마일개440안기산잔기적다태。 ZmCIPK42구유CIPK기인가족전형적격매결구역화NAF조공결구역，기안기산서렬여옥미CIPK15(NP_001108478)、수도CIP-K14(Q2QYM3.1)、소맥CIPK14(AFR90220.1)화대맥CIPK14(AEZ51505)등고등식물적CIPK안기산서렬적동원성분별위74%、73%、73%화70%。여의남개CIPK가족계통진화분석표명ZmCIPK42여의남개CIPK2화CIPK10동원관계교근。형광정량PCR분석발현ZmCIPK42기인재염협박화열처리후표체량현저승고，탈수처리후표체수평야유소제고，ABA화랭처리후표체량변화불명현。
According to the sequence of MaizeDGB database we cloned a maize CIPK42 gene (GRMZM-2G011919), ZmCIPK42 have an open reading frame (ORF) of 1 908 bp in length, which the full length cDNA is 1 323 bp, encoding a 440 amino acid residuals. ZmCIPK42 has the typical kinase domain and NAF regulatory domain of CIPK family. It's high homologous to other plants of CIPK as maize CIPK15 (NP_001108478) 74%, rice CIPK14 (Q2QYM3.1) 73%, wheat CIPK14 (AFR90220.1) and barley CIPK14 (AEZ51505). The phylogeny analysis showed that the ZmCIPK42 was close to CIPK2 and CIPK10 of Arabidopsis thaliana family. Real-time PCR showed that expression of ZmCIPK42 response to salt stress and heat treatment obviously, and expression level of dehydration treatment increased as well. It was not significantly to ABA and cold treatment.