中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2014年
30期
139-141
,共3页
贾先红%朱红梅%吴瑕%吕俊秀%郝传传
賈先紅%硃紅梅%吳瑕%呂俊秀%郝傳傳
가선홍%주홍매%오하%려준수%학전전
内毒素%肺纤维化%博来霉素%补肺通络%小鼠
內毒素%肺纖維化%博來黴素%補肺通絡%小鼠
내독소%폐섬유화%박래매소%보폐통락%소서
Endotoxin%Pulmonary fibrosis%Bleomycin%Tonifying lung and activating meridians%Mouse
目的：观察内毒素腹腔注射对小鼠急性肺损伤及肺纤维化的影响及其在中药干预下的变化。方法：10mg/kg 内毒素一次性腹腔注射制备小鼠急性肺损伤模型，造模即日起分别给予补肺通络中药或生理盐水灌胃，各组分别于d14、d28处死，观察不同处理条件下肺损伤及肺纤维化随时间的变化情况，HE染色观察肺组织形态学改变，Masson染色观察肺组织纤维化程度。结果：①d14时，给药组先于模型组进入损伤修复阶段，其肺组织纤维化评分（13.17±6.68）显著高于单纯模型组（2.38±1.06）（P<0.01），而肺损伤评分给药组（1.88±0.45）与模型组（1.70±0.31）尚无明显差别（P>0.05）；②d28时，模型组纤维化水平有较大幅度增加，而给药组变化则相对较小，模型组纤维化评分（18.44±2.65）与给药组（17.71±3.25）近似，二者间无统计学差异；两组小鼠肺组织炎症反应均有所消退，给药组基本接近正常而模型组尚存在较明显的炎症反应，肺损伤评分给药组（0.49±0.14）低于模型组（0.82±0.14）（P<0.01）。结论：内毒素能够替代博来霉素用以制备小鼠急性肺损伤肺纤维化模型。
目的：觀察內毒素腹腔註射對小鼠急性肺損傷及肺纖維化的影響及其在中藥榦預下的變化。方法：10mg/kg 內毒素一次性腹腔註射製備小鼠急性肺損傷模型，造模即日起分彆給予補肺通絡中藥或生理鹽水灌胃，各組分彆于d14、d28處死，觀察不同處理條件下肺損傷及肺纖維化隨時間的變化情況，HE染色觀察肺組織形態學改變，Masson染色觀察肺組織纖維化程度。結果：①d14時，給藥組先于模型組進入損傷脩複階段，其肺組織纖維化評分（13.17±6.68）顯著高于單純模型組（2.38±1.06）（P<0.01），而肺損傷評分給藥組（1.88±0.45）與模型組（1.70±0.31）尚無明顯差彆（P>0.05）；②d28時，模型組纖維化水平有較大幅度增加，而給藥組變化則相對較小，模型組纖維化評分（18.44±2.65）與給藥組（17.71±3.25）近似，二者間無統計學差異；兩組小鼠肺組織炎癥反應均有所消退，給藥組基本接近正常而模型組尚存在較明顯的炎癥反應，肺損傷評分給藥組（0.49±0.14）低于模型組（0.82±0.14）（P<0.01）。結論：內毒素能夠替代博來黴素用以製備小鼠急性肺損傷肺纖維化模型。
목적：관찰내독소복강주사대소서급성폐손상급폐섬유화적영향급기재중약간예하적변화。방법：10mg/kg 내독소일차성복강주사제비소서급성폐손상모형，조모즉일기분별급여보폐통락중약혹생리염수관위，각조분별우d14、d28처사，관찰불동처리조건하폐손상급폐섬유화수시간적변화정황，HE염색관찰폐조직형태학개변，Masson염색관찰폐조직섬유화정도。결과：①d14시，급약조선우모형조진입손상수복계단，기폐조직섬유화평분（13.17±6.68）현저고우단순모형조（2.38±1.06）（P<0.01），이폐손상평분급약조（1.88±0.45）여모형조（1.70±0.31）상무명현차별（P>0.05）；②d28시，모형조섬유화수평유교대폭도증가，이급약조변화칙상대교소，모형조섬유화평분（18.44±2.65）여급약조（17.71±3.25）근사，이자간무통계학차이；량조소서폐조직염증반응균유소소퇴，급약조기본접근정상이모형조상존재교명현적염증반응，폐손상평분급약조（0.49±0.14）저우모형조（0.82±0.14）（P<0.01）。결론：내독소능구체대박래매소용이제비소서급성폐손상폐섬유화모형。
Objective: To observe the impacts of endotoxin intraperitoneal injection on preparation of mouse acute pulmonary fibrosis mode and its changes under the intervention of Chinese medicine. Methods:Acute lung injury model of mice by intraperitoneal injection of 10mg/Kg lipopolysaccharide preparation, medicines on tonifying lung and activating meridians or saline were given to mice from the 1st day, rats in each group were killed on D14, D28. The different conditions of lung injury and pulmonary fibrosis were observed. the morphological changes were observed by lung tissue HE staining, and the degree of lung tissue fibrosis was observed by Masson staining . Results:On D14, the medicine group entered the damage repair phase earlier than the model group, its pulmonary fibrosis score (13.17±6.68) was significantly higher than that in the model group (2.38±1.06) (P<0.01), and the lung injury scores of the medicine group (1.88±0.45) and the model group (1.70±0.31) had no significantly difference (P>0.05);On D28, the level of fibrosis model group increased significantly, while the changes of the medicine group was relatively small, the fibrosis score of the model group (18.44±2.65) and medication group (17.71±3.25) were approximated with no statistical difference;the lung tissue inflammation of the two groups of mice had relived, the meditation group was basically close to the normal and the model groups still had obvious inflammatory reaction and lung injury score of the meditation group (0.49±0.14) was lower than that of the model group (0.82±0.14) (P<0.01). Conclusions:Endotoxin can replace bleomycin for the preparation of acute lung injury in mice model of pulmonary fibrosis.
