现代畜牧兽医
現代畜牧獸醫
현대축목수의
LIAONING JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY MEDICINE
2013年
7期
56-58
,共3页
林丽苗%周庆丰%余国莲%杜云平
林麗苗%週慶豐%餘國蓮%杜雲平
림려묘%주경봉%여국련%두운평
副猪嗜血杆菌(HPS)%培养基TSB%TSA
副豬嗜血桿菌(HPS)%培養基TSB%TSA
부저기혈간균(HPS)%배양기TSB%TSA
Haemophilus parasuis%Medium TSB%TSA
本试验对副猪嗜血杆菌的基础培养基成分、血清浓度、培养基pH值、保存条件进行优化筛选，旨在得出副猪嗜血杆菌的适宜培养保存条件，为副猪嗜血杆菌的发酵生产提供可靠的依据。通过试验得出，副猪嗜血杆菌的最佳培养保存条件：培养基起始pH值为7.3～7.5，成分为胰蛋白胨1.5%、大豆蛋白胨0.5%、NaCl 0.5%、酸水解酪蛋白0.5%、葡萄糖0.2%、5%新生牛血清和NAD 0.01%，用10%脱脂奶粉冻干保存在-20℃。
本試驗對副豬嗜血桿菌的基礎培養基成分、血清濃度、培養基pH值、保存條件進行優化篩選，旨在得齣副豬嗜血桿菌的適宜培養保存條件，為副豬嗜血桿菌的髮酵生產提供可靠的依據。通過試驗得齣，副豬嗜血桿菌的最佳培養保存條件：培養基起始pH值為7.3～7.5，成分為胰蛋白胨1.5%、大豆蛋白胨0.5%、NaCl 0.5%、痠水解酪蛋白0.5%、葡萄糖0.2%、5%新生牛血清和NAD 0.01%，用10%脫脂奶粉凍榦保存在-20℃。
본시험대부저기혈간균적기출배양기성분、혈청농도、배양기pH치、보존조건진행우화사선，지재득출부저기혈간균적괄의배양보존조건，위부저기혈간균적발효생산제공가고적의거。통과시험득출，부저기혈간균적최가배양보존조건：배양기기시pH치위7.3～7.5，성분위이단백동1.5%、대두단백동0.5%、NaCl 0.5%、산수해락단백0.5%、포도당0.2%、5%신생우혈청화NAD 0.01%，용10%탈지내분동간보존재-20℃。
Based on the experiment of medium composition, serum concentration, pH value of medium, preservation conditions of Haemophilus parasuis were optimized, getting the suitable culture conditions and providing the reliable basis for the fermentation production of Haemophilus parasuis. The conclusion was: the best cul-ture conditions and preservation of Haemophilus parasuis are as follows: the medi-um initial pH value was 7.3~7.5, medium composition for tryptone peptone 1.5%, soy peptone 0.5%, NaCl 0.5%, acid hydrolyzed casein 0.5%, glucose 0.2%, fetal bovine serum 5%, NAD 0.01%, freeze drying at the temperature of -20 ℃ with 10% skim milk powder.
