包装与食品机械
包裝與食品機械
포장여식품궤계
PACKAGING AND FOOD MACHINERY
2013年
3期
13-17
,共5页
李同刚%陈康健%洪鹏志%杨萍
李同剛%陳康健%洪鵬誌%楊萍
리동강%진강건%홍붕지%양평
罗非鱼下脚料%酶解%超滤%螯合
囉非魚下腳料%酶解%超濾%螯閤
라비어하각료%매해%초려%오합
Tilapia scraps%enzymolysis%ultrafiltration%chelation
以罗非鱼下脚料为原料,通过正交试验优化中性蛋白酶与风味蛋白酶的复合酶解条件,并对酶解液进行超滤,比较不同超滤组分的螯合效果,利用紫外吸收和傅立叶红外扫描对螯合盐进行了鉴定。结果表明:复合酶水解条件为温度55℃、加酶量1500 U/g、复合比1∶2、固液比1∶4、自然pH值;水解度为14.43%酶解液的螯合率为77.92%,其超滤组分中,10 KDa以下组分的螯合效果最好,螯合率达到89.36%,1 KDa以下组分的螯合率较低为61.45%。
以囉非魚下腳料為原料,通過正交試驗優化中性蛋白酶與風味蛋白酶的複閤酶解條件,併對酶解液進行超濾,比較不同超濾組分的螯閤效果,利用紫外吸收和傅立葉紅外掃描對螯閤鹽進行瞭鑒定。結果錶明:複閤酶水解條件為溫度55℃、加酶量1500 U/g、複閤比1∶2、固液比1∶4、自然pH值;水解度為14.43%酶解液的螯閤率為77.92%,其超濾組分中,10 KDa以下組分的螯閤效果最好,螯閤率達到89.36%,1 KDa以下組分的螯閤率較低為61.45%。
이라비어하각료위원료,통과정교시험우화중성단백매여풍미단백매적복합매해조건,병대매해액진행초려,비교불동초려조분적오합효과,이용자외흡수화부립협홍외소묘대오합염진행료감정。결과표명:복합매수해조건위온도55℃、가매량1500 U/g、복합비1∶2、고액비1∶4、자연pH치;수해도위14.43%매해액적오합솔위77.92%,기초려조분중,10 KDa이하조분적오합효과최호,오합솔체도89.36%,1 KDa이하조분적오합솔교저위61.45%。
The composite enzymatic hydrolysis conditions of neutral protease and flavourzyme were opti -mized by orthogonal test ,the ultrafiltration of hydrolysate from tilapia scraps was done to compare chelating ac-tivity,and chelating salt was identified by UV-Visible Spectrophotometer and Fourier Transform Infrared Spec-trometer.The results showed that the optimal conditions were temperature 55℃,enzyme concentration 1500 U/g,composite ratio 1∶2,solid-liquid ratio1∶4,natural pH.The chelation rate of enzymatic hydrolysate reached 77 .92%, hydrolysis degree was 14 .43%.Among these ultrafiltrate fractions of the hydrolysate , the compo-nents,which molecular weight were less than 10KDa,showed the highest zinc-binding capacity,the chelation rate as high as 89.36%,while the fractions below 1KDa had a relatively lower rate of 61.45%.