嘉应学院学报
嘉應學院學報
가응학원학보
JOURNAL OF JIAYING UNIVERSITY
2012年
5期
61-66
,共6页
铅胁迫%叶片%生理指标
鉛脅迫%葉片%生理指標
연협박%협편%생리지표
lead stress%leaf%physiological characteristics
以红树植物桐花树2a生龄苗为试验材料,采用淡水水培法研究了不同浓度Pb2+(0mg/L(对照组)、10mg/L、25mg/L、50mg/L、100mg/L、300mg/L、500mg/L)对桐花树叶片的超氧负离子(02。)、丙二醛(MDA)含量、过氧化物酶(POD)与超氧化物歧化酶(SOD)活性、蛋白质以及可溶性总糖量含量的影响.结果表明:随着Pb2+浓度的升高,桐花树叶片中O2一和MDA含量均呈上升趋势,且均显著高于对照,最高值范围是在Pb2+浓度100~500mg/L;而蛋白质含量和POD的活性则呈下降趋势,且均显著低于对照.SOD活性在铅胁迫浓度为10mg/L时达到最大,当高于10mg/L时,则随Pb2+浓度的升高而下降.可溶性糖含量在Pb2+浓度为0-300mg/L时,随Pb2+浓度的升高而显著增大.试验表明:桐花树叶片在浓度为10—100mg/L铅处理下,可以通过自身的内在调控抵御铅污染,即轻度的铅污染有利于桐花树的生长;在300—500mg/L高浓度铅胁迫下,各种生理指标出现衰减,显示高浓度铅胁迫的抑制效应,不利于桐花树生长.
以紅樹植物桐花樹2a生齡苗為試驗材料,採用淡水水培法研究瞭不同濃度Pb2+(0mg/L(對照組)、10mg/L、25mg/L、50mg/L、100mg/L、300mg/L、500mg/L)對桐花樹葉片的超氧負離子(02。)、丙二醛(MDA)含量、過氧化物酶(POD)與超氧化物歧化酶(SOD)活性、蛋白質以及可溶性總糖量含量的影響.結果錶明:隨著Pb2+濃度的升高,桐花樹葉片中O2一和MDA含量均呈上升趨勢,且均顯著高于對照,最高值範圍是在Pb2+濃度100~500mg/L;而蛋白質含量和POD的活性則呈下降趨勢,且均顯著低于對照.SOD活性在鉛脅迫濃度為10mg/L時達到最大,噹高于10mg/L時,則隨Pb2+濃度的升高而下降.可溶性糖含量在Pb2+濃度為0-300mg/L時,隨Pb2+濃度的升高而顯著增大.試驗錶明:桐花樹葉片在濃度為10—100mg/L鉛處理下,可以通過自身的內在調控牴禦鉛汙染,即輕度的鉛汙染有利于桐花樹的生長;在300—500mg/L高濃度鉛脅迫下,各種生理指標齣現衰減,顯示高濃度鉛脅迫的抑製效應,不利于桐花樹生長.
이홍수식물동화수2a생령묘위시험재료,채용담수수배법연구료불동농도Pb2+(0mg/L(대조조)、10mg/L、25mg/L、50mg/L、100mg/L、300mg/L、500mg/L)대동화수협편적초양부리자(02。)、병이철(MDA)함량、과양화물매(POD)여초양화물기화매(SOD)활성、단백질이급가용성총당량함량적영향.결과표명:수착Pb2+농도적승고,동화수협편중O2일화MDA함량균정상승추세,차균현저고우대조,최고치범위시재Pb2+농도100~500mg/L;이단백질함량화POD적활성칙정하강추세,차균현저저우대조.SOD활성재연협박농도위10mg/L시체도최대,당고우10mg/L시,칙수Pb2+농도적승고이하강.가용성당함량재Pb2+농도위0-300mg/L시,수Pb2+농도적승고이현저증대.시험표명:동화수협편재농도위10—100mg/L연처리하,가이통과자신적내재조공저어연오염,즉경도적연오염유리우동화수적생장;재300—500mg/L고농도연협박하,각충생리지표출현쇠감,현시고농도연협박적억제효응,불리우동화수생장.
Two - year - old plants of Aegiceras comiculatum cultivated in fresh water were exposed to different con- centrations of Pb2+ (0 mg/L( CK), 10mg/L,25 mg/L,50 mg/L, 100 mg/L,300mg/L and 500 mg/L) to investigate the effect of lead exposure on the contents of superoxide radical ( 02 - ), malonie dialdehyde ( MDA), peroxidase (POD) and superoxide dismutase (SOD), protein and soluble sugar. Results indicated that, with the increase of Pb2+, 02 - and MDA increased to significantly higher than those under control, and reached the highest values un- der Pb2+100 - 500mg/L; whereas protein content and POD activity declined significantly as Pb2+ increased. SOD activity reached the highest value at 10 mg/L Pb2+ , and decreased with raised Pb2+ concentrations. Soluble sugar content significantly increased with the increase of Pb2+ within the range of 0 - 300 mg/L. The conclusion is that: exposure of Pb2+ at 10 - 100mg/L stimulated growth of Aegiceras comiculatum,indicating there existed an intrinsic adaptive mechanism to light lead stress in the plant; but as concentration of lead increased to 300 - 500mg/L, growth of Aegiceras comiculatum was significantly inhibited, with all physiological indexes significantly declined.