微生物与感染
微生物與感染
미생물여감염
JOURNAL OF MICROBES AND INFECTION
2013年
4期
227-233
,共7页
邵俊杰%王嘉瑜%滕峥%潘浩%陈洪友%匡小舟%俞雪莲%朱兆奎%张曦
邵俊傑%王嘉瑜%滕崢%潘浩%陳洪友%劻小舟%俞雪蓮%硃兆奎%張晞
소준걸%왕가유%등쟁%반호%진홍우%광소주%유설련%주조규%장희
肠道病毒%人肠道病毒71型%手足口病%上海
腸道病毒%人腸道病毒71型%手足口病%上海
장도병독%인장도병독71형%수족구병%상해
Enterovirus%Human enterovirus 71%Hand,foot and mouth disease%Shanghai
为分析2009~2011年上海地区手足口病流行病学特征和病原构成,从国家疾病监测信息报告管理系统获取上海市2009~2011年手足口病流行病学资料;采用实时荧光反转录聚合酶链反应(RT-PCR )对来自上海18个区(县)的6676例手足口病病例标本进行肠道病毒核酸检测,对其中257份标本进行病毒分离;对27份人肠道病毒71型(HEV71)毒株进行VP1基因序列全长测定和分析。结果显示,2009~2011年上海市18个区(县)均有手足口病病例报道,地区分布无显著差异;≤5岁的婴幼儿为疾病高发年龄段;4~11月为发病高峰期。HEV71和柯萨奇病毒 A组16型(CA16)为主要病原,不同地区病原构成有所不同。实时荧光RT-PCR对6676例病例标本进行核酸检测,其中肠道病毒通用核酸检测阳性率为69.61%,HEV71和CA16阳性率分别为38.83%和21.26%。对257份HEV71核酸阳性标本进行病毒分离,获得毒株57株,分离阳性率为22.18%。对其中27株进行VP1基因全长测序,均属C4a基因亚型。
為分析2009~2011年上海地區手足口病流行病學特徵和病原構成,從國傢疾病鑑測信息報告管理繫統穫取上海市2009~2011年手足口病流行病學資料;採用實時熒光反轉錄聚閤酶鏈反應(RT-PCR )對來自上海18箇區(縣)的6676例手足口病病例標本進行腸道病毒覈痠檢測,對其中257份標本進行病毒分離;對27份人腸道病毒71型(HEV71)毒株進行VP1基因序列全長測定和分析。結果顯示,2009~2011年上海市18箇區(縣)均有手足口病病例報道,地區分佈無顯著差異;≤5歲的嬰幼兒為疾病高髮年齡段;4~11月為髮病高峰期。HEV71和柯薩奇病毒 A組16型(CA16)為主要病原,不同地區病原構成有所不同。實時熒光RT-PCR對6676例病例標本進行覈痠檢測,其中腸道病毒通用覈痠檢測暘性率為69.61%,HEV71和CA16暘性率分彆為38.83%和21.26%。對257份HEV71覈痠暘性標本進行病毒分離,穫得毒株57株,分離暘性率為22.18%。對其中27株進行VP1基因全長測序,均屬C4a基因亞型。
위분석2009~2011년상해지구수족구병류행병학특정화병원구성,종국가질병감측신식보고관리계통획취상해시2009~2011년수족구병류행병학자료;채용실시형광반전록취합매련반응(RT-PCR )대래자상해18개구(현)적6676례수족구병병례표본진행장도병독핵산검측,대기중257빈표본진행병독분리;대27빈인장도병독71형(HEV71)독주진행VP1기인서렬전장측정화분석。결과현시,2009~2011년상해시18개구(현)균유수족구병병례보도,지구분포무현저차이;≤5세적영유인위질병고발년령단;4~11월위발병고봉기。HEV71화가살기병독 A조16형(CA16)위주요병원,불동지구병원구성유소불동。실시형광RT-PCR대6676례병례표본진행핵산검측,기중장도병독통용핵산검측양성솔위69.61%,HEV71화CA16양성솔분별위38.83%화21.26%。대257빈HEV71핵산양성표본진행병독분리,획득독주57주,분리양성솔위22.18%。대기중27주진행VP1기인전장측서,균속C4a기인아형。
The present paper aims to investigate the epidemiological and etiological characteristics of hand , foot and mouth disease (HFMD) in Shanghai from 2009 to 2011 .The data from the National Notifiable Disease Surveillance System were analyzed .A total of 6 676 clinical samples were collected and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to determine the pathogens .The results showed that 18 districts/counties had reported HFMD cases ;The children ≤5 years old were the most susceptible population ;The peak of HFMD epidemic was from April to November ;Human enterovirus 71 (HEV71 ) and coxsackievirus A16 (CA16 ) were the major pathogens for this epidemic , but the constituent ratio of HEV71 and CA16 was different in different months and regions . Out of the 6 676 patients ,4 647 cases were positive for enterovirus by real-time RT-PCR ,The positive rate was 69 .61% . Among them 1 419 were CA16 (the overall positive rate of 21 .26% ) and 2 592 were HEV71 (the overall positive rate of 38 .83% ) .257 HEV71 samples were cultured with human rhabdomyosarcoma (RD) cells , and 57 HEV71 strains were obtained and 27 of these were subjected to sequence analysis . The complete sequences of VP1-encoding region in several identified HEV71 strains were analyzed . BLAST sequence analysis showed that all the 27 isolated HEV71 strains from 2009 to 2011 were classified into C4a .