CAJ | 학술논문

生物安全实验室微环境消毒是控制实验室污染的重要环节。过氧化氢广泛用于病原微生物实验室微环境消毒，但其对不同病原微生物的消毒效果有待研究。本文研究了过氧化氢干雾（粒径＜10μm ）以不同消毒程序对生物安全柜表面常见微生物的消毒效果。结果显示，在生物安全柜内采用优化的消毒程序（发散循环8次，每次1 min ，达60 ppm后，静置消毒2 h ），过氧化氢干雾可完全杀灭1×106 CFU枯草芽胞杆菌、嗜热脂肪芽胞杆菌、金黄色葡萄球菌、表皮葡萄球菌、耻垢分枝杆菌，以及1×108 CFU大肠埃希菌。然而，当金黄色葡萄球菌、表皮葡萄球菌、耻垢分枝杆菌浓度达1×107 CFU时，过氧化氢干雾无法完全杀灭。因此，建议在进行过氧化氢干雾消毒时，应先用消毒剂处理，以期彻底杀灭生物安全柜微环境中污染的病原微生物。
생물안전실험실미배경소독시공제실험실오염적중요배절。과양화경엄범용우병원미생물실험실미배경소독，단기대불동병원미생물적소독효과유대연구。본문연구료과양화경간무（립경＜10μm ）이불동소독정서대생물안전거표면상견미생물적소독효과。결과현시，재생물안전거내채용우화적소독정서（발산순배8차，매차1 min ，체60 ppm후，정치소독2 h ），과양화경간무가완전살멸1×106 CFU고초아포간균、기열지방아포간균、금황색포도구균、표피포도구균、치구분지간균，이급1×108 CFU대장애희균。연이，당금황색포도구균、표피포도구균、치구분지간균농도체1×107 CFU시，과양화경간무무법완전살멸。인차，건의재진행과양화경간무소독시，응선용소독제처리，이기철저살멸생물안전거미배경중오염적병원미생물。
Disinfection of microenvironment surface is very important for control contamination of biosafety laboratory .Hydrogen peroxide has been used widely in biosafety laboratories for disinfection .The effect of hydrogen peroxide on inactivation of different bacteria was investigated in the present study .The effect of hydrogen peroxide in the form of dry-mist (diameter <10 μm) on sterilization of common pathogens in the biosafety cabinet (BSC) was evaluated .The optimized procedure (diffusing 1 min for 8 cycles ,reaching 60 ppm ,and staying 2 h) has been determined ,at which hydrogen peroxide dry-mist was able to completely kill 6 logs of Staphylococcus aureus (S . aureus) ,Staphylococcus epidermidis (S .epidermidis) ,Mycobacterium smegmatis (M .smegmatis) ,Bacillus subtilis (B .subtilis) and Bacillus stearothermophilus (B .stearother-mophilus) ,as well as 8 logs of Escherichia coli (E .coli) ,but not 7 logs of S .aureus ,S .epidermidis and M .smegmatis .Therefore ,it is suggested that before disinfection of BSC surface microenvironment by hydrogen peroxide dry-mist it is necessary to wipe the BSC surface or decontaminate spillages with disinfect-ants .