CAJ | 학술논문

结合室内与室外培养，设置雷州马尾藻（Sargassum leizhouense）雌雄种藻质量比分别为2∶1，4∶1，5∶1和6∶1，幼孢子体密度分别为1，2，5，10，15，20和30株/mL，研究了不同雌雄种藻质量比对采苗量及其存活率的影响以及不同密度培养幼孢子体的相对生长率及存活率。结果显示：雷州马尾藻雌雄种藻质量比为4∶1时，单位质量雌体的产苗量最大，达6604．3株/g，单位面积苗帘的附苗量最大，达63．5株/cm2。经过40 d培育，苗帘上幼孢子体存活率以雌雄种藻质量比为5∶1组最高，达56．2％，而4∶1组最低，仅20．9％。在试验范围内，培养密度对幼孢子体生长的影响不显著（P＞0．05），而显著影响其存活率（P＜0．05）。室内培养21 d后，30株/cm2组幼孢子体存活率最低，为87．5％，与其它密度组有显著差异（P＜0．05）。因此，雷州马尾藻人工采苗适宜的雌雄种藻质量比为5∶1，有利于其幼孢子体生长、存活的适宜密度为＜30株/cm2。
결합실내여실외배양，설치뇌주마미조（Sargassum leizhouense）자웅충조질량비분별위2∶1，4∶1，5∶1화6∶1，유포자체밀도분별위1，2，5，10，15，20화30주/mL，연구료불동자웅충조질량비대채묘량급기존활솔적영향이급불동밀도배양유포자체적상대생장솔급존활솔。결과현시：뇌주마미조자웅충조질량비위4∶1시，단위질량자체적산묘량최대，체6604．3주/g，단위면적묘렴적부묘량최대，체63．5주/cm2。경과40 d배육，묘렴상유포자체존활솔이자웅충조질량비위5∶1조최고，체56．2％，이4∶1조최저，부20．9％。재시험범위내，배양밀도대유포자체생장적영향불현저（P＞0．05），이현저영향기존활솔（P＜0．05）。실내배양21 d후，30주/cm2조유포자체존활솔최저，위87．5％，여기타밀도조유현저차이（P＜0．05）。인차，뇌주마미조인공채묘괄의적자웅충조질량비위5∶1，유리우기유포자체생장、존활적괄의밀도위＜30주/cm2。
Combining indoor and outdoor culture , four groups of the mass ratio of parental female to male fronds (2∶1, 4∶1, 5∶1, 6∶1) and seven groups of juvenile sporophytes density (1,2, 5, 10, 15, 20, and 30/mL) of Sargassum leizhouense were set up , to study the effect of different mass ratio of parental female to male fronds of S.leizhouense on seedling amount adhering to seedling curtains and their survival rate .And the relative growth rate and survival rate of cultured juvenile sporophytes at different cultural density were measured.The results showed that 6 604.3 individuals, the most amount of seedlings per mass of female frond, and 63.5 seedlings of per milliliter on seedling curtain were obtained at mass ratio of 4:1(♀∶♂). However , after 40-day outside culture , the highest seedling survival rate was 56.2 percent at mass ratio of 5∶1 (♀∶♂) , while it was lowest ( only 20 .9 percent ) at mass ratio of 4∶1 (♀∶♂) .There was no significant difference for effect of cultural density on the growth of juvenile sporophytes ( P>0.05 ) , but on the growth rate(P<0.05).The lowest survival rate (87.5%) seedling was obtained at density of 30/cm2 after 21-day indoor culture .There was significant difference from other density groups ( P <0 .05 ) .In conclusion , the optimum mass ratio of parental female to male fronds for artificial collection of S.leizhouense seedlings is 5∶1, and the suitable culture density for growth and survival of juvenile S .l eizhouense seedlings is less than 30/cm2.