临床合理用药杂志
臨床閤理用藥雜誌
림상합리용약잡지
CHINESE JOURNAL OF CLINICAL RATIONAL DRUG USE
2014年
18期
23-24
,共2页
胰岛素样生长因子Ⅱ%软骨细胞%基质金属蛋白酶1%骨关节炎
胰島素樣生長因子Ⅱ%軟骨細胞%基質金屬蛋白酶1%骨關節炎
이도소양생장인자Ⅱ%연골세포%기질금속단백매1%골관절염
Insulin-like growth factorⅡ%Chondrocytes%Matrix metalloproteinase 1%Osteoarthroitis
目的：研究胰岛素样生长因子Ⅱ（ IGFⅡ）对软骨细胞基质金属蛋白酶1（ MMP-1）基因表达调节的机制。方法采用体外新生大鼠股骨髁软骨细胞培养，同时加入不同剂量白介素-1β（ IL-1β），使用酶联免疫吸附技术检测体外培养的关节软骨细胞上清液24h、48h、72h中基因蛋白MMP-1mRNA的表达情况，并加入IGFⅡ干预，观察IGFⅡ的效果。结果 IL-1β可增加关节软骨细胞MMP-1基因蛋白的分泌，加入IGFⅡ干预后可减少关节软骨细胞MMP-1基因蛋白的分泌。结论 IGFⅡ具有显著促进关节软骨细胞增殖的效果。
目的：研究胰島素樣生長因子Ⅱ（ IGFⅡ）對軟骨細胞基質金屬蛋白酶1（ MMP-1）基因錶達調節的機製。方法採用體外新生大鼠股骨髁軟骨細胞培養，同時加入不同劑量白介素-1β（ IL-1β），使用酶聯免疫吸附技術檢測體外培養的關節軟骨細胞上清液24h、48h、72h中基因蛋白MMP-1mRNA的錶達情況，併加入IGFⅡ榦預，觀察IGFⅡ的效果。結果 IL-1β可增加關節軟骨細胞MMP-1基因蛋白的分泌，加入IGFⅡ榦預後可減少關節軟骨細胞MMP-1基因蛋白的分泌。結論 IGFⅡ具有顯著促進關節軟骨細胞增殖的效果。
목적：연구이도소양생장인자Ⅱ（ IGFⅡ）대연골세포기질금속단백매1（ MMP-1）기인표체조절적궤제。방법채용체외신생대서고골과연골세포배양，동시가입불동제량백개소-1β（ IL-1β），사용매련면역흡부기술검측체외배양적관절연골세포상청액24h、48h、72h중기인단백MMP-1mRNA적표체정황，병가입IGFⅡ간예，관찰IGFⅡ적효과。결과 IL-1β가증가관절연골세포MMP-1기인단백적분비，가입IGFⅡ간예후가감소관절연골세포MMP-1기인단백적분비。결론 IGFⅡ구유현저촉진관절연골세포증식적효과。
Objective To investigate the regulating effects of insulin-like growth factorⅡ( IGFⅡ) on gene expres-sion of matrix metalloproteinases( MMP) -1 in chondrocytes. Methods The condyles of femur chondrocytes of 7 days rats were harvested and cultured with different doses of interleukin( IL) -1β,the monolayer cell cultured of chondrocyte MMP-1 mRNA level were assessed by the enzyme-linked immunosorbent at 24th,48th and 72th hours,harvested and cultured with IGFⅡ. Effect of IGFⅡ was observed. Results IL-1β increased the MMP-1mRNA level respectively in cultures of chondro-cytes,IGFⅡdecreased the MMP-1mRNA level respectively in cultures of chondrocytes. Conclusion IGFⅡ can promote chondrocytes proliferation obviously.
