实用医技杂志
實用醫技雜誌
실용의기잡지
JOURNAL OF PRACTICAL MEDICAL TECHNIQUES
2014年
4期
360-362
,共3页
蛛网膜下腔出血%血管痉挛,颅内%血管紧张素Ⅱ%表没食子儿茶素没食子酸酯%内皮素
蛛網膜下腔齣血%血管痙攣,顱內%血管緊張素Ⅱ%錶沒食子兒茶素沒食子痠酯%內皮素
주망막하강출혈%혈관경련,로내%혈관긴장소Ⅱ%표몰식자인다소몰식자산지%내피소
Subarachnoid hemorrhage%Vasospasm,intracranial%Angiotensin-Ⅱ%Epigallocatechin gallate%Endothelin
目的:研究表没食子儿茶素没食子酸酯(EGCG)对大鼠蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)的作用并探索其作用机制。方法将24只SD大鼠采用随机数字表法分为4组,即空白组、0.9%氯化钠注射液组、SAH组、SAH+EGCG组,苏木素-伊红(HE)染色观察各组大鼠基底动脉管径的变化,反转录-聚合酶链反应(RT-PCR)定量检测各组大鼠血管内皮细胞中血管紧张素Ⅱ(AngⅡ)及内皮素(ET)基因水平。结果 HE染色结果提示SAH组和SAH+EGCG组基底动脉管壁厚度及管腔狭窄程度显著大于空白组、0.9%氯化钠注射液组,而SAH+EGCG组显著<SAH组,差异具有统计学意义(P<0.05);SAH组和SAH+EGCG组内皮细胞AngⅡ及ET的mRNA表达水平显著高于空白组及0.9%氯化钠注射液组(P<0.05),而SAH+EGCG组血管却低于SAH组,差异具有统计学意义(P<0.05)。结论 EGCG能够降低SAH后血管内皮细胞AngⅡ及ET基因水平及其表达,可能具有一定的抗血管痉挛作用。
目的:研究錶沒食子兒茶素沒食子痠酯(EGCG)對大鼠蛛網膜下腔齣血(SAH)後腦血管痙攣(CVS)的作用併探索其作用機製。方法將24隻SD大鼠採用隨機數字錶法分為4組,即空白組、0.9%氯化鈉註射液組、SAH組、SAH+EGCG組,囌木素-伊紅(HE)染色觀察各組大鼠基底動脈管徑的變化,反轉錄-聚閤酶鏈反應(RT-PCR)定量檢測各組大鼠血管內皮細胞中血管緊張素Ⅱ(AngⅡ)及內皮素(ET)基因水平。結果 HE染色結果提示SAH組和SAH+EGCG組基底動脈管壁厚度及管腔狹窄程度顯著大于空白組、0.9%氯化鈉註射液組,而SAH+EGCG組顯著<SAH組,差異具有統計學意義(P<0.05);SAH組和SAH+EGCG組內皮細胞AngⅡ及ET的mRNA錶達水平顯著高于空白組及0.9%氯化鈉註射液組(P<0.05),而SAH+EGCG組血管卻低于SAH組,差異具有統計學意義(P<0.05)。結論 EGCG能夠降低SAH後血管內皮細胞AngⅡ及ET基因水平及其錶達,可能具有一定的抗血管痙攣作用。
목적:연구표몰식자인다소몰식자산지(EGCG)대대서주망막하강출혈(SAH)후뇌혈관경련(CVS)적작용병탐색기작용궤제。방법장24지SD대서채용수궤수자표법분위4조,즉공백조、0.9%록화납주사액조、SAH조、SAH+EGCG조,소목소-이홍(HE)염색관찰각조대서기저동맥관경적변화,반전록-취합매련반응(RT-PCR)정량검측각조대서혈관내피세포중혈관긴장소Ⅱ(AngⅡ)급내피소(ET)기인수평。결과 HE염색결과제시SAH조화SAH+EGCG조기저동맥관벽후도급관강협착정도현저대우공백조、0.9%록화납주사액조,이SAH+EGCG조현저<SAH조,차이구유통계학의의(P<0.05);SAH조화SAH+EGCG조내피세포AngⅡ급ET적mRNA표체수평현저고우공백조급0.9%록화납주사액조(P<0.05),이SAH+EGCG조혈관각저우SAH조,차이구유통계학의의(P<0.05)。결론 EGCG능구강저SAH후혈관내피세포AngⅡ급ET기인수평급기표체,가능구유일정적항혈관경련작용。
Objective To study the effect of epigallocatechin gallate(EGCG) on cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH) and research the mechanism. Methods Twenty-four SD rats were randomly divided into 4 groups, namely control group, saline group, SAH group, SAH+EGCG group, HE staining of the basilar artery, and RT-PCR were used to detect the expression of angiotensin Ⅱ(AngⅡ) and endothelin(ET) in vascular endothelial cells. Results Basilar artery wall thickening and luminal stenosis in SAH group and SAH+EGCG group was statistically significant than control, saline group, SAH+EGCG group was more significant compared with SAH group(P<0.05); the expression of AngⅡ and ET′s mRNA in SAH group and SAH+EGCG group was greater than control and saline group , furthermore, SAH+EGCG group was statistically less than SAH group (P<0.05). Conclusion EGCG can reduce the expression of AngⅡand ET in vascular endothelial cells and attenuate CVS after SAH.