CAJ | 학술논문

基于磷酸二酯酶（PDE）对细菌纤维素（BC）合成产生抑制的机理，对细菌纤维素生产菌株醋酸杆菌（Acetobacter xylinum）的PDE编码基因（pde基因）进行了基因敲除研究，构建PDE失活型（PDE -）重组菌株，并通过氨苄青霉素及氯霉素抗生素培养基筛选出目的重组菌株。发酵及遗传稳定性实验结果表明，PDE -重组菌株发酵生产BC产量比出发菌株最大可提高38％，且遗传性能稳定。本研究为提高BC发酵产量奠定了基础。
기우린산이지매（PDE）대세균섬유소（BC）합성산생억제적궤리，대세균섬유소생산균주작산간균（Acetobacter xylinum）적PDE편마기인（pde기인）진행료기인고제연구，구건PDE실활형（PDE -）중조균주，병통과안변청매소급록매소항생소배양기사선출목적중조균주。발효급유전은정성실험결과표명，PDE -중조균주발효생산BC산량비출발균주최대가제고38％，차유전성능은정。본연구위제고BC발효산량전정료기출。
According to the mechanism that the phosphodiesterase(PDE) would restrain the synthesis of bacterial cellulose(BC) ,pde-gene which code PDE in the Acetobacter xylinum producing BC is disrupted ,and new strains named PDE - strain w hose PDE have non-activity are constructed .The aim strains are filtrated using the culture medium which contains ampi-cillin and chloromycetin .The results of the fermentation shows that the BC output of PDE -strain can increased by 38% than the starting strain ,and its heredity was stable .This study has laid certain foundation to increase the fermentation output of BC .