CAJ | 학술논문

血管紧张素转化酶（ACE）抑制肽在人体血压调节方面有着重要的作用，它常被用于治疗高血压及其他相关疾病，降低患者的发病率及死亡率．本文通过单因素试验研究了温度、pH、酶添加量、底物浓度以及反应时间对Alcalase酶解羊乳酪蛋白制备ACE抑制肽的水解度及ACE抑制率的影响，结果表明各因素的最适条件为：温度55℃、pH 7．0、酶添加量E/S为5％、底物浓度为10％、水解时间为120 min ，ACE抑制率和水解度分别为89．09％及18．91％．
혈관긴장소전화매（ACE）억제태재인체혈압조절방면유착중요적작용，타상피용우치료고혈압급기타상관질병，강저환자적발병솔급사망솔．본문통과단인소시험연구료온도、pH、매첨가량、저물농도이급반응시간대Alcalase매해양유락단백제비ACE억제태적수해도급ACE억제솔적영향，결과표명각인소적최괄조건위：온도55℃、pH 7．0、매첨가량E/S위5％、저물농도위10％、수해시간위120 min ，ACE억제솔화수해도분별위89．09％급18．91％．
Angiotensin-converting enzyme (ACE) inhibitory peptides play an important role in the regulation of blood pressure .It is usually used in therapy to reduce morbidity and mortal-ity of patients with hypertension and other related diseases .In the present research , the effect of process conditions on the ACE inhibitory peptides activity from goat′s milk casein hydrolysed with Alcalase was investigated .It was shown that ACE inhibitory activity of goat′s milk casein hydrolysates could be controlled by regulation of five process conditions (hydrolysis temperature ,pH and enzyme to substrate (E/S) ratio ,substrate concentration , hydrolysis time) .Temperature at 55 ℃ ,pH 7 .0 ,E/S ratio at 5% ,substrate concentration at 10% and hydrolysis time 120 min were found to be the optimal conditions to obtain high ACE inhibitory activity 89 .09% and degree of hydrolysis 18 .91% .