CAJ | 학술논문

目的：建立一种快速、特异、灵敏的鸭源性成分检测方法。方法本研究以鸭线粒体基因全序列为靶位点设计引物和探针，进行荧光定量PCR扩增，建立鸭源性成分检测方法；以常见畜禽肉包括羊肉、牛肉、鸡肉、鹅肉、猪肉、兔肉、马肉、鹿肉等参考动物物种作特异性检测；以50 mg/kg羊肉DNA作为稀释液对鸭肉DNA进行梯度稀释，做灵敏度检测。结果该方法能够有效对鸭源性成分进行快速检测，具有较强的特异性，灵敏度较高(可达0.1μg/kg)并且羊肉成分的存在对鸭肉灵敏度检测没有影响。结论该方法特异性强，灵敏度高，可以快速、准确检测畜肉食品中含有的鸭源性成分。
목적：건립일충쾌속、특이、령민적압원성성분검측방법。방법본연구이압선립체기인전서렬위파위점설계인물화탐침，진행형광정량PCR확증，건립압원성성분검측방법；이상견축금육포괄양육、우육、계육、아육、저육、토육、마육、록육등삼고동물물충작특이성검측；이50 mg/kg양육DNA작위희석액대압육DNA진행제도희석，주령민도검측。결과해방법능구유효대압원성성분진행쾌속검측，구유교강적특이성，령민도교고(가체0.1μg/kg)병차양육성분적존재대압육령민도검측몰유영향。결론해방법특이성강，령민도고，가이쾌속、준학검측축육식품중함유적압원성성분。
Objective To establish a rapid, specific, sensitive method for detection of duck derived materials. Methods The real-time PCR method for detection of duck-derived materials in meat products was established using forward and reverse primers and probe corresponding to the mitochondrial gene. The specific detection was carried out by livestock and poultry meat including mutton, beef, chicken, goose, por-cine, rabbit meat, horse meat, venison and other reference animal species. Sensitivity detection was done using the 50 mg/kg mutton DNA as diluent for gradient dilution of duck DNA. Results This method can effectively detect duck derived materials, with a strong specificity and high sensitivity (up to 0.1μg/kg), and without obvious influence by mutton components on duck sensitivity detection. Conclusion This method has a strong specificity and high sensitivity, and can rapidly and accurately detect the duck-derived compo-nents in meat products.