CAJ | 학술논문

目的：为提高黄瓜采后炭疽病病原菌葫芦科刺盘孢Colletotrichum orbiculare的识别效率，探究该病原菌的侵染对黄瓜采后贮藏品质的影响。方法本文针对黄瓜炭疽病病原葫芦科刺盘孢 Colletotrichum orbiculare 的ITS序列设计特异性引物，以黄瓜自然发病状态下分离的疑似黄瓜炭疽病病原菌DNA为模板，利用PCR反应进行基因克隆，并将所得的目的片段进行测序后，确定为黄瓜炭疽病病原菌葫芦科刺盘孢 Colletotrichum orbiculare。将病原菌反接于黄瓜，取发病腐烂组织，测定硬度、可滴定酸含量、可溶性固形物含量指标。结果结果表明，黄瓜炭疽病病原会造成黄瓜硬度降低，可滴定酸含量升高，可溶性固形物含量降低，严重影响黄瓜的采后贮藏品质。结论本文为黄瓜采后炭疽病病原菌的分子识别提供参考方法，并未针对黄瓜采后炭疽病病原菌Colletotrichum orbiculare的控制措施提供研究基础。
목적：위제고황과채후탄저병병원균호호과자반포Colletotrichum orbiculare적식별효솔，탐구해병원균적침염대황과채후저장품질적영향。방법본문침대황과탄저병병원호호과자반포 Colletotrichum orbiculare 적ITS서렬설계특이성인물，이황과자연발병상태하분리적의사황과탄저병병원균DNA위모판，이용PCR반응진행기인극륭，병장소득적목적편단진행측서후，학정위황과탄저병병원균호호과자반포 Colletotrichum orbiculare。장병원균반접우황과，취발병부란조직，측정경도、가적정산함량、가용성고형물함량지표。결과결과표명，황과탄저병병원회조성황과경도강저，가적정산함량승고，가용성고형물함량강저，엄중영향황과적채후저장품질。결론본문위황과채후탄저병병원균적분자식별제공삼고방법，병미침대황과채후탄저병병원균Colletotrichum orbiculare적공제조시제공연구기출。
Objective In order to improve the identify efficiency of cucumber postharvest anthracnose pathogen Colletotrichum orbiculare and explore the effect of the pathogenic bacteria's infection on postharvest storage quality of cucumber influence. Methods Specific primers were designed based on the ITS sequence of anthracnose pathogen (Colletotrichum orbiculare) in cucumber. The DNA of suspected anthracnose pathogen that separated from the invaded cucumber was taken as the DNA template and the PCR reaction was conducted for gene cloning. The acquired target DNA sequence was analytically sequenced and identified as anthracnose pathogen (Colletotrichum orbiculare). The pathogen was inoculated reversely into cucumber, and the rotten tissue was separated from the cucumber. The indexes of hardness, titratable acidity content, and soluble solid content were determined. Results The results showed that the anthracnose pathogen in cucumber could cause the decrease of hardness and soluble solid content, increase of titratable acidity content, which seriously influenced postharvest storage quality of cucumber. Conclusion So we provide the reference for cucumber postharvest anthracnose pathogen molecular recognition method and the basis for cucumber postharvest anthracnose pathogen Colletotrichum orbiculare control measures.