CAJ | 학술논문

目的：通过诱变方法获得高效降解亚硝酸盐的优良乳酸菌应用于降低腌制品中的亚硝酸盐。方法以前期实验筛选获得的降解亚硝酸盐性能较强乳酸菌 D2作为初始诱变菌株,采用紫外线和亚硝基胍复合诱变,选育高效降解亚硝酸盐的乳酸菌。结果经15 W紫外线和0.5 mg/mL亚硝基胍三轮复合诱变得到一株优良乳酸菌,该菌株24 h降解亚硝酸盐(200 mg/L)降解率为91.4%,较初始菌株提高了12.7%；以亚硝酸钠为底物,其产亚硝酸盐还原酶的比活力为7.7 mmol/L,较诱变前提高42.9%；连续传代培养后降解亚硝酸盐能力和产亚硝酸盐还原酶活力性能稳定。结论通过紫外线和亚硝基胍复合诱变,获得一株遗传稳定性良好的高效降解亚硝酸盐的菌株。
목적：통과유변방법획득고효강해아초산염적우량유산균응용우강저업제품중적아초산염。방법이전기실험사선획득적강해아초산염성능교강유산균 D2작위초시유변균주,채용자외선화아초기고복합유변,선육고효강해아초산염적유산균。결과경15 W자외선화0.5 mg/mL아초기고삼륜복합유변득도일주우량유산균,해균주24 h강해아초산염(200 mg/L)강해솔위91.4%,교초시균주제고료12.7%；이아초산납위저물,기산아초산염환원매적비활력위7.7 mmol/L,교유변전제고42.9%；련속전대배양후강해아초산염능력화산아초산염환원매활력성능은정。결론통과자외선화아초기고복합유변,획득일주유전은정성량호적고효강해아초산염적균주。
Objective To obtain efficient nitrite degrading lactic acid bacteria by mutagenesis. Methods With the strain D2 isolated in the preliminary work as the original strain, the UV/NTG mutagenesis were used to prepare for screening. Results After mutagenesis with 15 W UV and 0.5 mg/mL nitrosoguanidine for three times, the new strain obtained degradated 91.4% nitrite (200 mg/L) after cultivation for 24 h, increasing by 12.7%compared with the original. With sodium nitrite as the substrate, the activity of nitrite reductase enzyme reached 7.7 mmol/L, increasing by 42.9%. The ability of nitrite degradation and the activity of nitrite reductase were stable after several generations. Conclusion A high quality nitrite degrading strain stable genetic cha-racteristics was obtained via UV/NTG mutagenesis.