大连医科大学学报
大連醫科大學學報
대련의과대학학보
JOURNAL OF DALIAN MEDICAL UNIVERSITY
2013年
6期
543-546
,共4页
魏明丽%丁怀玉%吕田%李真%于红玖%王梅%马裴裴
魏明麗%丁懷玉%呂田%李真%于紅玖%王梅%馬裴裴
위명려%정부옥%려전%리진%우홍구%왕매%마배배
晚期氧化蛋白产物%基质细胞衍生因子-1α%NF-κB
晚期氧化蛋白產物%基質細胞衍生因子-1α%NF-κB
만기양화단백산물%기질세포연생인자-1α%NF-κB
advanced oxidation protein products%stromal cell derived factor -1α%NF-κB
目的：观察晚期氧化蛋白产物（ advanced oxidation protein product ，AOPP）对内皮细胞表达基质细胞衍生因子-1α（stromal cell derived factor -1α，SDF-1α）的影响，并探讨其作用机制。方法 ECV304细胞分别经0，50，100，200μmol/L AOPP处理后，用RT-PCR法检测其SDF-1αmRNA的表达，观察AOPP对ECV304细胞的SDF-1α表达的影响。 ECV304细胞先经NF-κB通路特异性阻断剂BAY11-7082处理，再经AOPP处理，用RT-PCR法检测其SDF-1αmRNA的表达，观察BAY11-7082对AOPP促ECV304细胞SDF-1αmRNA表达作用的影响。结果 AOPP促进ECV304细胞SDF-1αmRNA 表达，并且随AOPP浓度的升高，SDF-1αmRNA 的表达增加。对照组、AOPP 50μmol/L组、AOPP 100μmol/L组及AOPP 200μmol/L组SDF-1αmRNA/GAPDH mRNA值分别为0．034±0．005、0．109±0．019、0．226±0．037及0．322±0．043，组间比较差异均有显著性意义，P＜0．05。BAY11-7082抑制AOPP促ECV304细胞SDF-1αmRNA表达，两组细胞SDF-1αmRNA/GAPDH mRNA值分别为0．256±0．035和0．322±0．043，P＜0．05。结论 AOPP可上调ECV304细胞表达SDF-1α，NF-κB通路可能是介导这一作用的重要途径之一。
目的：觀察晚期氧化蛋白產物（ advanced oxidation protein product ，AOPP）對內皮細胞錶達基質細胞衍生因子-1α（stromal cell derived factor -1α，SDF-1α）的影響，併探討其作用機製。方法 ECV304細胞分彆經0，50，100，200μmol/L AOPP處理後，用RT-PCR法檢測其SDF-1αmRNA的錶達，觀察AOPP對ECV304細胞的SDF-1α錶達的影響。 ECV304細胞先經NF-κB通路特異性阻斷劑BAY11-7082處理，再經AOPP處理，用RT-PCR法檢測其SDF-1αmRNA的錶達，觀察BAY11-7082對AOPP促ECV304細胞SDF-1αmRNA錶達作用的影響。結果 AOPP促進ECV304細胞SDF-1αmRNA 錶達，併且隨AOPP濃度的升高，SDF-1αmRNA 的錶達增加。對照組、AOPP 50μmol/L組、AOPP 100μmol/L組及AOPP 200μmol/L組SDF-1αmRNA/GAPDH mRNA值分彆為0．034±0．005、0．109±0．019、0．226±0．037及0．322±0．043，組間比較差異均有顯著性意義，P＜0．05。BAY11-7082抑製AOPP促ECV304細胞SDF-1αmRNA錶達，兩組細胞SDF-1αmRNA/GAPDH mRNA值分彆為0．256±0．035和0．322±0．043，P＜0．05。結論 AOPP可上調ECV304細胞錶達SDF-1α，NF-κB通路可能是介導這一作用的重要途徑之一。
목적：관찰만기양화단백산물（ advanced oxidation protein product ，AOPP）대내피세포표체기질세포연생인자-1α（stromal cell derived factor -1α，SDF-1α）적영향，병탐토기작용궤제。방법 ECV304세포분별경0，50，100，200μmol/L AOPP처리후，용RT-PCR법검측기SDF-1αmRNA적표체，관찰AOPP대ECV304세포적SDF-1α표체적영향。 ECV304세포선경NF-κB통로특이성조단제BAY11-7082처리，재경AOPP처리，용RT-PCR법검측기SDF-1αmRNA적표체，관찰BAY11-7082대AOPP촉ECV304세포SDF-1αmRNA표체작용적영향。결과 AOPP촉진ECV304세포SDF-1αmRNA 표체，병차수AOPP농도적승고，SDF-1αmRNA 적표체증가。대조조、AOPP 50μmol/L조、AOPP 100μmol/L조급AOPP 200μmol/L조SDF-1αmRNA/GAPDH mRNA치분별위0．034±0．005、0．109±0．019、0．226±0．037급0．322±0．043，조간비교차이균유현저성의의，P＜0．05。BAY11-7082억제AOPP촉ECV304세포SDF-1αmRNA표체，량조세포SDF-1αmRNA/GAPDH mRNA치분별위0．256±0．035화0．322±0．043，P＜0．05。결론 AOPP가상조ECV304세포표체SDF-1α，NF-κB통로가능시개도저일작용적중요도경지일。
Objective To observe the effect of advanced oxidation protein product ( AOPP) on the expression of SDF-1αin ECV304 cells,and to investigate the possible mechanism .Methods SDF-1αmRNA expressions were revealed by RT -PCR respectively in ECV304 cells preincubated with different concentrations of AOPP .SDF-1αmRNA expression was re-vealed by RT-PCR in ECV304 cells preincubated with BAY 11-7082 which was a inhibitor of NF -κB signal transduc-tion pathway .Results SDF-1αexpression was constitutionally detected in ECV 304 cells and was up-regulated by AOPP in a concentration-dependent manner .The promoting effect of AOPP on the expression of SDF -1αin ECV304 cells could be inhibited by BAY11-7082.Conclusion AOPP could up-regulate SDF-1αexpression in ECV304 cells by NF-κB signal transduction pathway .
