大连医科大学学报
大連醫科大學學報
대련의과대학학보
JOURNAL OF DALIAN MEDICAL UNIVERSITY
2013年
6期
533-537
,共5页
顾金萍%于健%王之余%曲茂兴
顧金萍%于健%王之餘%麯茂興
고금평%우건%왕지여%곡무흥
脓毒症%阿托伐他汀%C反应蛋白(CRP)%大鼠
膿毒癥%阿託伐他汀%C反應蛋白(CRP)%大鼠
농독증%아탁벌타정%C반응단백(CRP)%대서
sepsis%atorvastatin%C response protein%rat
目的：观察阿托伐他汀对脓毒症大鼠血中C反应蛋白（ CRP）浓度的影响，探讨他汀类药物是否影响宿主炎症反应。方法选择健康雄性S-D（ Sprague-Dawley）大鼠100只，采用盲肠结扎法（ CLP）制造大鼠脓毒症模型，随机分为对照组、脓毒症组、阿托伐他汀低剂量组和阿托伐他汀高剂量组，各组25只。于术后0 h，3 h，6 h，12 h和24 h采血，分离血浆检测CRP的浓度。观察和比较各组大鼠活动度，小肠组织病理改变。采用SPSS13．0软件包，于各时间点行重复测量资料方差分析。结果0 h各组大鼠血浆CRP浓度比较差异无显著性意义（P＞0．05）。对照组血浆CRP浓度在各时间点变化不明显，在3 h、6 h、12 h、24 h阿托伐他汀低剂量组、阿托伐他汀高剂量组的血浆CRP浓度较脓毒症组和对照组比较差异均有显著性意义，明显低于脓毒症组（P＜0．01），阿托伐他汀高剂量组较阿托伐他汀低剂量组血浆CRP浓度降低更明显（P＜0．01）。脓毒症组大鼠脓毒症表现最强，阿托伐他汀组脓毒症表现明显减轻，高剂量组较低剂量组减轻更为明显，对照组没有相关表现。结论阿托伐他汀能抑制脓毒症大鼠CRP释放，降低血浆CRP的水平。
目的：觀察阿託伐他汀對膿毒癥大鼠血中C反應蛋白（ CRP）濃度的影響，探討他汀類藥物是否影響宿主炎癥反應。方法選擇健康雄性S-D（ Sprague-Dawley）大鼠100隻，採用盲腸結扎法（ CLP）製造大鼠膿毒癥模型，隨機分為對照組、膿毒癥組、阿託伐他汀低劑量組和阿託伐他汀高劑量組，各組25隻。于術後0 h，3 h，6 h，12 h和24 h採血，分離血漿檢測CRP的濃度。觀察和比較各組大鼠活動度，小腸組織病理改變。採用SPSS13．0軟件包，于各時間點行重複測量資料方差分析。結果0 h各組大鼠血漿CRP濃度比較差異無顯著性意義（P＞0．05）。對照組血漿CRP濃度在各時間點變化不明顯，在3 h、6 h、12 h、24 h阿託伐他汀低劑量組、阿託伐他汀高劑量組的血漿CRP濃度較膿毒癥組和對照組比較差異均有顯著性意義，明顯低于膿毒癥組（P＜0．01），阿託伐他汀高劑量組較阿託伐他汀低劑量組血漿CRP濃度降低更明顯（P＜0．01）。膿毒癥組大鼠膿毒癥錶現最彊，阿託伐他汀組膿毒癥錶現明顯減輕，高劑量組較低劑量組減輕更為明顯，對照組沒有相關錶現。結論阿託伐他汀能抑製膿毒癥大鼠CRP釋放，降低血漿CRP的水平。
목적：관찰아탁벌타정대농독증대서혈중C반응단백（ CRP）농도적영향，탐토타정류약물시부영향숙주염증반응。방법선택건강웅성S-D（ Sprague-Dawley）대서100지，채용맹장결찰법（ CLP）제조대서농독증모형，수궤분위대조조、농독증조、아탁벌타정저제량조화아탁벌타정고제량조，각조25지。우술후0 h，3 h，6 h，12 h화24 h채혈，분리혈장검측CRP적농도。관찰화비교각조대서활동도，소장조직병리개변。채용SPSS13．0연건포，우각시간점행중복측량자료방차분석。결과0 h각조대서혈장CRP농도비교차이무현저성의의（P＞0．05）。대조조혈장CRP농도재각시간점변화불명현，재3 h、6 h、12 h、24 h아탁벌타정저제량조、아탁벌타정고제량조적혈장CRP농도교농독증조화대조조비교차이균유현저성의의，명현저우농독증조（P＜0．01），아탁벌타정고제량조교아탁벌타정저제량조혈장CRP농도강저경명현（P＜0．01）。농독증조대서농독증표현최강，아탁벌타정조농독증표현명현감경，고제량조교저제량조감경경위명현，대조조몰유상관표현。결론아탁벌타정능억제농독증대서CRP석방，강저혈장CRP적수평。
Objective To investigate the influences of atorvastatin on C -response protein (CRP) of rats with sepsis.To discuss whether the statins would affect the host response to inflammation .Methods Sepsis models were establishing using 100 male SD rats.The rats were manufactured models by cecal ligation and puncture (CLP).A total of 100 healthy rats randomly were divided into 4 groups (n=25): Sham -operation group, CLP group, low-dose atorvastatin group and high-dose atorvastatin group.Blood samples of five rats in each group were collected at postoperative 0, 3, 6, 12 and 24 hours to detect CRP in plasma .We observed and compared the activity of rats in each group .Specimens of small intestine were taken for pathological examination by optical microscope .Results At 0 h, plasma CRP levels were not significantly different among the groups .CRP levels in low -dose and high -dose atorvastatin groups were both significantly lower at postoperative 3, 6, 12 and 24 hours (P<0.01) than those in CLP group.CRP levels in high -dose atorvastatin group were significantly lower than those in low -dose group (P<0.01) at the four time points.Conclusion Atorvastatin could inhibit the expression of CRP in blood plasma of sepsis rats ,and might have anti -septic effect .
