CAJ | 학술논문

目的：观察大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用。方法采用四氯化碳灌胃法建立肝纤维化大鼠模型，随机分成对照组、肝纤维化组、空白纳米颗粒组（ SPIO组）、大豆异黄酮组（ SI组）、载药纳米颗粒组（ SI-SPIO组），每组24只。对照组给予生理盐水尾静脉注射，其他各组除四氯化碳灌胃外，分别给予生理盐水尾静脉注射、SPIO、大豆异黄酮和SI-SPIO载药纳米颗粒尾静脉注射治疗，观察对照组和治疗组之间肝脏生化指标、过氧化损伤指标和肝脏组织学变化。结果8周后对各组大鼠的肝脏生化指标和肝纤维化程度进行评估，SI组和SI-SPIO组明显优于肝纤维化组和SPIO组；过氧化指标检测，与对照组相比，肝纤维化组和SPIO组MDA明显升高（2．45±0．77，2．69±1．01，P＜0．05），GSH -Px 和SOD明显降低（300．74±16．25，299．18±15．57和409．77±20．46，431．56±19．25，P＜0．05）；与肝纤维化组相比，SI组和SI-SPIO组MDA明显降低（0．85±0．12，0．90±0．02，P＜0．05），GSH-Px和SOD明显升高（340．25±11．85，366．45±14．83和467．27±16．11，485．75±22．54，P＜0．05）。 SI-SPIO组过氧化指标较SI组改善更为明显（P＜0．05）。结论大豆异黄酮具有抗氧化作用，能够抑制大鼠肝纤维化形成，载药纳米有助于药效的发挥。
목적：관찰대두이황동재약납미과립대간섬유화대서적항과양화작용。방법채용사록화탄관위법건립간섬유화대서모형，수궤분성대조조、간섬유화조、공백납미과립조（ SPIO조）、대두이황동조（ SI조）、재약납미과립조（ SI-SPIO조），매조24지。대조조급여생리염수미정맥주사，기타각조제사록화탄관위외，분별급여생리염수미정맥주사、SPIO、대두이황동화SI-SPIO재약납미과립미정맥주사치료，관찰대조조화치료조지간간장생화지표、과양화손상지표화간장조직학변화。결과8주후대각조대서적간장생화지표화간섬유화정도진행평고，SI조화SI-SPIO조명현우우간섬유화조화SPIO조；과양화지표검측，여대조조상비，간섬유화조화SPIO조MDA명현승고（2．45±0．77，2．69±1．01，P＜0．05），GSH -Px 화SOD명현강저（300．74±16．25，299．18±15．57화409．77±20．46，431．56±19．25，P＜0．05）；여간섬유화조상비，SI조화SI-SPIO조MDA명현강저（0．85±0．12，0．90±0．02，P＜0．05），GSH-Px화SOD명현승고（340．25±11．85，366．45±14．83화467．27±16．11，485．75±22．54，P＜0．05）。 SI-SPIO조과양화지표교SI조개선경위명현（P＜0．05）。결론대두이황동구유항양화작용，능구억제대서간섬유화형성，재약납미유조우약효적발휘。
Objective To investigate the anti -peroxidative effects of SPIO nanoparticles as a drug delivery system when combined with soybean isoflavones ( SI-SPIO ) .Methods The liver fibrosis models of rats were established by CCL 4 ga-vage.120 rats were random grouped as control , fibrosis, SPIO tail vein injection, isoflavone treatment and SI -SPIO treat-ment group .Normal saline injections were given in control group , CCL4 gavage were given in other groups , as well as NS injection, SPIO, SI and SI-SPIO injections to 24 rats of each group, respectively.Biochemical and histological indicators were analyzed .Peroxidative indicators were detected as well .Results Liver fibrosis was significantly improved in SI and SI-SPIO groups vs .control both in biochemical and histological aspects .Peroxcidative indicators such as MDA increased in liver fibrotic and SPIO groups (2.45 ±0.77, 2.69 ±1.01, P<0.05), GSH -Px and SOD decreased (300.74 ± 16.25, 299.18 ±15.57 and 409.77 ±20.46, 431.56 ±19.25, P<0.05), SI administration reduced MDA (0.85 ± 0.12,0.90 ±0.02, P<0.05), increased GSH -Px and SOD in SI and SI -SPIO groups(340.25 ±11.85, 366.45 ± 14.83 and 467.27 ±16.11, 485.75 ±22.54, P<0.05).SI-SPIO group presents much more efficiency comparing with SI group (P<0.05).Conclusion SI has anti-peroxidative effect and inhibits liver fibrosis in rats .SPIO nanoparticles make the effects more efficient as a drug delivery system .