CAJ | 학술논문

目的探讨和研究hsa-miR-340表达上调对人膀胱癌T24细胞增殖、凋亡能力的影响。方法化学合成hsa-miR-340模拟物，瞬时转染膀胱癌T24细胞，以实时荧光定量聚合酶链反应（RT-PCR）检测hsa-miR-340在膀胱癌T24细胞中相对表达量，确定能够转染成功并有较高的转染率后，分别通过四甲基偶氮唑蓝（MTT）法和流式细胞计数仪，分析转染hsa-miR-340模拟物前后对膀胱癌T24细胞的增殖及凋亡能力的影响。结果①hsa-miR-340模拟物可在膀胱癌T24细胞中表达，并以RT-PCR验证相对含量较高，差异有统计学意义（P＜0.05）。②增殖实验中分别在48h和72h时把实验组与阴性对照组和空白对照组相对比，膀胱癌T24细胞生长增殖明显受抑制且差异有统计学意义（P＜0.05），说明hsa-miR-340过表达后，能使T24细胞增殖能力受到有效抑制。③hsa-miR-340模拟物转染T24细胞48h后，以流式细胞仪检测T24细胞凋亡率，分别为实验组（11.37±0.41）％、阴性对照组（6.72±0.32）％、空白对照组（6.62±0.23）％，比较后差异有统计学意义（P＜0.05），说明hsa-miR-340过表达使T24细胞的凋亡显著增加。结论hsa-miR-340可能参与膀胱癌的进展。
목적탐토화연구hsa-miR-340표체상조대인방광암T24세포증식、조망능력적영향。방법화학합성hsa-miR-340모의물，순시전염방광암T24세포，이실시형광정량취합매련반응（RT-PCR）검측hsa-miR-340재방광암T24세포중상대표체량，학정능구전염성공병유교고적전염솔후，분별통과사갑기우담서람（MTT）법화류식세포계수의，분석전염hsa-miR-340모의물전후대방광암T24세포적증식급조망능력적영향。결과①hsa-miR-340모의물가재방광암T24세포중표체，병이RT-PCR험증상대함량교고，차이유통계학의의（P＜0.05）。②증식실험중분별재48h화72h시파실험조여음성대조조화공백대조조상대비，방광암T24세포생장증식명현수억제차차이유통계학의의（P＜0.05），설명hsa-miR-340과표체후，능사T24세포증식능력수도유효억제。③hsa-miR-340모의물전염T24세포48h후，이류식세포의검측T24세포조망솔，분별위실험조（11.37±0.41）％、음성대조조（6.72±0.32）％、공백대조조（6.62±0.23）％，비교후차이유통계학의의（P＜0.05），설명hsa-miR-340과표체사T24세포적조망현저증가。결론hsa-miR-340가능삼여방광암적진전。
Objective To investigate effects of upregulated hsa-miR-340 on the proliferation and apoptosis of bladder cancer T24 cells .Methods Hsa-miR-340 mimics were chemically synthetized and transiently transfect-ed into bladder cancer T24 cells .The expression of hsa-miR-340 in T24 cells was determined using quantitative polymerase chain reaction (RT-PCR) .Make sure that the T24 cells had been successfully transfected and on a high rate .Proliferation and apoptosis of the T24 cells were respectively evaluated using flow cytometry (FCM ) and MTT assay .Results ① The bladder cancer T24 cells could be transfected by hsa-miR-340 mimics and hsa-miR-340 quantification showed high expression in T24 cells (P<0.05) .②Compared with the control group and the blank group ,the experimental group could significantly inhibit proliferation of T 24 cells (P<0.05) .③In the FCM test ,we put each group into the flow cytometric and found that the apoptosis rates of the experimental group ,the control group and the blank group were (11.37 ± 0.41)% ,(6.72 ± 0.32)% and (6.62 ± 0.23)% ,re-spectively .The apoptosis of T24 cells was obviously increased (P<0.05) .Conclusion The relative high expres-sion of hsa-miR-340 may be involved in the progression of bladder cancer .