CAJ | 학술논문

通过对鼠李糖乳杆菌利用碳源、氮源能力的研究,探讨菌株产酸的最佳发酵条件。结果表明：高质量浓度葡萄糖对菌体的生长和乳酸发酵有一定的抑制作用;酵母粉是鼠李糖乳杆菌生长和产L-乳酸最理想的氮源,使用廉价的氮源黄豆粉和无机氮源硫酸铵替代培养基中的酵母粉,可以使产酸量基本不变。在接种量5%的情况下,以酵母粉为氮源,当葡萄糖初始质量浓度为60mg/mL时,在恒温厌氧箱培养48h,L-乳酸产量为48.25mg/mL,产率为80.53%。
통과대서리당유간균이용탄원、담원능력적연구,탐토균주산산적최가발효조건。결과표명：고질량농도포도당대균체적생장화유산발효유일정적억제작용;효모분시서리당유간균생장화산L-유산최이상적담원,사용렴개적담원황두분화무궤담원류산안체대배양기중적효모분,가이사산산량기본불변。재접충량5%적정황하,이효모분위담원,당포도당초시질량농도위60mg/mL시,재항온염양상배양48h,L-유산산량위48.25mg/mL,산솔위80.53%。
The abilities of Lactobacillus rhamnosus to utilize various carbon and nitrogen sources were examined with the aim of finding optimal carbon and nitrogen sources for lactic acid production by Lactobacillus rhamnosus.It was indicated that glucose was the best carbon source although it at high concentrations had a certain inhibitory effect on Lactobacillus rhamnosus growth and lactic acid production.Yeast extract was the best nitrogen carbon for Lactobacillus rhamnosus growth and lactic acid production.Basically the same lactic acid production was obtained when yeast extract was replaced with other cheap nitrogen carbons such as soybean meal or ammonium sulfate.The lactic acid yield and productivity were 48.25 mg/mL and 80.53%,respectively after 48 h of culture in a medium containing yeast extract as the nitrogen source and glucose as the carbon nitrogen at an initial concentration of 60 mg/mL under thermostatic anaerobic conditions.