五邑大学学报：自然科学版
五邑大學學報：自然科學版
오읍대학학보：자연과학판
Journal of Wuyi University(Natural Science Edition)
2012年
3期
35-41
,共7页
李辰%吴盼盼%卿宁%梁硕%黄俊添
李辰%吳盼盼%卿寧%樑碩%黃俊添
리신%오반반%경저%량석%황준첨
蛹虫草%培养残基%虫草素%微波%超声波
蛹蟲草%培養殘基%蟲草素%微波%超聲波
용충초%배양잔기%충초소%미파%초성파
cordyceps%deserted solid medium%cordycepin%microwaves%ultrasonic waves
优化并比较了微波辅助和超声波辅助提取蛹虫草培养残基中虫草素的工艺方法.采用L 9（34）正交试验设计,考察了料液比（g：mL）、乙醇含量（%）、微波功率（微波法）或提取温度（超声波法）、提取时间等4个因素对虫草素提取率的影响.结果表明：微波提取以25倍料液比、40%乙醇、240 W下微波提取1.5 min,提取2次为最佳工艺,该条件下浸膏得率为28.33%,虫草素含量为0.378 mg/g;超声波提取在45 kHz功率下,以25倍料液比、40%乙醇、60℃下超声提取30 min,提取2次为最佳工艺,该条件下浸膏得率为38.24%,虫草素含量为0.362 mg/g.比较了超声波提取2次、微波提取2次、微波—超声波联合提取以及超声—微波联合提取等方式对虫草素的提取效率,最终确定微波提取2次为最佳工艺,该工艺适用于蛹虫草培养残基中虫草素的快速高效提取.
優化併比較瞭微波輔助和超聲波輔助提取蛹蟲草培養殘基中蟲草素的工藝方法.採用L 9（34）正交試驗設計,攷察瞭料液比（g：mL）、乙醇含量（%）、微波功率（微波法）或提取溫度（超聲波法）、提取時間等4箇因素對蟲草素提取率的影響.結果錶明：微波提取以25倍料液比、40%乙醇、240 W下微波提取1.5 min,提取2次為最佳工藝,該條件下浸膏得率為28.33%,蟲草素含量為0.378 mg/g;超聲波提取在45 kHz功率下,以25倍料液比、40%乙醇、60℃下超聲提取30 min,提取2次為最佳工藝,該條件下浸膏得率為38.24%,蟲草素含量為0.362 mg/g.比較瞭超聲波提取2次、微波提取2次、微波—超聲波聯閤提取以及超聲—微波聯閤提取等方式對蟲草素的提取效率,最終確定微波提取2次為最佳工藝,該工藝適用于蛹蟲草培養殘基中蟲草素的快速高效提取.
우화병비교료미파보조화초성파보조제취용충초배양잔기중충초소적공예방법.채용L 9（34）정교시험설계,고찰료료액비（g：mL）、을순함량（%）、미파공솔（미파법）혹제취온도（초성파법）、제취시간등4개인소대충초소제취솔적영향.결과표명：미파제취이25배료액비、40%을순、240 W하미파제취1.5 min,제취2차위최가공예,해조건하침고득솔위28.33%,충초소함량위0.378 mg/g;초성파제취재45 kHz공솔하,이25배료액비、40%을순、60℃하초성제취30 min,제취2차위최가공예,해조건하침고득솔위38.24%,충초소함량위0.362 mg/g.비교료초성파제취2차、미파제취2차、미파—초성파연합제취이급초성—미파연합제취등방식대충초소적제취효솔,최종학정미파제취2차위최가공예,해공예괄용우용충초배양잔기중충초소적쾌속고효제취.
Microwave-assisted extraction （MAE） extracting cordycepin from deserted solid medium and ultrasonic-assisted extraction （UAE） for of Cordyceps were optimized and compared. The solvent quantum to solid medium （g：mL）, ethanol content （%）, microwave power （W） （in MAE） or extraction temperature （~C） （in UAE） and extraction time （rain）, etc, were reviewed using the L9（34） orthogonal design test. The extraction conditions of MAE and UAE method were both optimized. The results showed that the optimum process conditions were as follows： adding 25 folds of 40% ethanol, extracting for 1.5 min under 240 W power in MAE, two times, the extraction yield of cordycepin from the deserted solid medium was 0.378 mg/g with extractum yield at 28.33% in this process; adding 25folds of 40% ethanol, extracting for 30 min at 60 ~C in UAE, two times, the extraction yield of cordycepin from the deserted solid medium was 0.362 mg/g with extractum yield at 38.24% in this process. The MAE, UAE and eombined extraction of MAE and UAE were all developed and compared, and MAE was confirmed and chosen as the optimal method for more efficient, simpler and faster extraction of cordycepin. This developed technology is proper for the rapid and efficient extraction of cordycepin from discarded solid medium of cuhured Cordyceps.
