浙江海洋学院学报：自然科学版
浙江海洋學院學報：自然科學版
절강해양학원학보：자연과학판
Journal of Zhejiang Ocean University(Natural Science Edition)
2012年
3期
197-201
,共5页
吴一挺%吕敢堂%任夙艺%王志铮%曹彪
吳一挺%呂敢堂%任夙藝%王誌錚%曹彪
오일정%려감당%임숙예%왕지쟁%조표
水温%存活率%耗氧率%鳃组织%CAT%SOD%日本沼虾
水溫%存活率%耗氧率%鰓組織%CAT%SOD%日本沼蝦
수온%존활솔%모양솔%새조직%CAT%SOD%일본소하
water temperature%survival rate%consumption rate%gill tissue%CAT%SOD%Macrobrachium mipponensis
在pH 7.11±0.07,基准水温24.0±0.2℃条件下,采用静水停食法开展了水温对日本沼虾（体长4.29±0.32 cm,体质量1.80±0.12 g）存活、耗氧率及鳃组织CAT、SOD酶活力的影响实验。结果表明（：1）48 h内水温12.5℃和32.5℃实验组日本沼虾的死亡率均超过50%,而15～30℃范围内的存活率则为100%（;2）水温22.5～27.5℃范围内,日本沼虾昼均、夜均及各观测时段耗氧率均随水温升高而增大（P〈0.05）,但耗氧高峰时段和耗氧昼夜节律均未发生改变;（3）48 h内,水温22.5℃和25℃实验组日本沼虾鳃组织SOD活力时序变化步调一致且无差异（P〈0.05）,各时段观测值均低于27.5℃实验组（P〈0.05）,低谷期较27.5℃实验组后延12 h;（4）水温22.5～27.5℃日本沼虾鳃组织CAT活力在48 h内均较为稳定,且均随水温升高而显著增大（P〈0.05）。
在pH 7.11±0.07,基準水溫24.0±0.2℃條件下,採用靜水停食法開展瞭水溫對日本沼蝦（體長4.29±0.32 cm,體質量1.80±0.12 g）存活、耗氧率及鰓組織CAT、SOD酶活力的影響實驗。結果錶明（：1）48 h內水溫12.5℃和32.5℃實驗組日本沼蝦的死亡率均超過50%,而15～30℃範圍內的存活率則為100%（;2）水溫22.5～27.5℃範圍內,日本沼蝦晝均、夜均及各觀測時段耗氧率均隨水溫升高而增大（P〈0.05）,但耗氧高峰時段和耗氧晝夜節律均未髮生改變;（3）48 h內,水溫22.5℃和25℃實驗組日本沼蝦鰓組織SOD活力時序變化步調一緻且無差異（P〈0.05）,各時段觀測值均低于27.5℃實驗組（P〈0.05）,低穀期較27.5℃實驗組後延12 h;（4）水溫22.5～27.5℃日本沼蝦鰓組織CAT活力在48 h內均較為穩定,且均隨水溫升高而顯著增大（P〈0.05）。
재pH 7.11±0.07,기준수온24.0±0.2℃조건하,채용정수정식법개전료수온대일본소하（체장4.29±0.32 cm,체질량1.80±0.12 g）존활、모양솔급새조직CAT、SOD매활력적영향실험。결과표명（：1）48 h내수온12.5℃화32.5℃실험조일본소하적사망솔균초과50%,이15～30℃범위내적존활솔칙위100%（;2）수온22.5～27.5℃범위내,일본소하주균、야균급각관측시단모양솔균수수온승고이증대（P〈0.05）,단모양고봉시단화모양주야절률균미발생개변;（3）48 h내,수온22.5℃화25℃실험조일본소하새조직SOD활력시서변화보조일치차무차이（P〈0.05）,각시단관측치균저우27.5℃실험조（P〈0.05）,저곡기교27.5℃실험조후연12 h;（4）수온22.5～27.5℃일본소하새조직CAT활력재48 h내균교위은정,차균수수온승고이현저증대（P〈0.05）。
The thesis carried out the research about the effect of survival rate, consumption rate and CAT, SOD enzyme activity of gill tissue from water temperature on Macrobrachium mipponensis （body lenth 0.32 cm, body weigt 1.80＋0.12 g）, on condition that pH 7.11＋0.07, benchmark water temperature at 24.0＋0.2℃, in stagnant watehr and no feeding. The result showed that： （1） the death rate exceeded 50% in the groups when water temperature were 12.5 ℃ and 32.5℃, yet the death rate was 100% in the groups which water tempera- ture were 15-30 ℃ within 48 h on M. mipponensis; （2） the consumption rate of daily average, and night aver- age both amplified with water temperature raised during 22.5-27.5 ℃ water temperature, yet neither peak pe- riod nor rhythm of consumption had changes; （3） the change rhythm of SOD enzyme activity showed synchronous and no difference between 22.5℃group and 25 ℃ group, and both under 27.5 ℃ group every ob- serving time interval （P〈0.05）, low peak period of 22.5 ℃ group and 25 ~C group delay 12 h compared with 27.5 ~C group. （4） CAT enzyme activity of gill tissue showed unconverted, and amplified with water temperature rising significantly, during 22.5-27.5 ℃ water temperature within 48 h about M. mipponensis.
