山东科学
山東科學
산동과학
SHANDONG SCIENCE
2012年
4期
22-26
,共5页
杨合同%陈凯%李纪顺%周红姿%赵晓燕
楊閤同%陳凱%李紀順%週紅姿%趙曉燕
양합동%진개%리기순%주홍자%조효연
黑蛋巢菌属%红蛋巢菌属%几丁质酶%β-1,3-葡聚糖酶%纤维素酶%木聚糖酶%木质素过氧化物酶
黑蛋巢菌屬%紅蛋巢菌屬%幾丁質酶%β-1,3-葡聚糖酶%纖維素酶%木聚糖酶%木質素過氧化物酶
흑단소균속%홍단소균속%궤정질매%β-1,3-포취당매%섬유소매%목취당매%목질소과양화물매
Cyathus spp.%Nidula spp.%chitinase%β-1,3-Glucanase%cellulase%xylanase%lignin peroxidase
通过测定黑蛋巢菌属9个菌株和红蛋巢菌属6个菌株的几丁质酶、β-1,3-葡聚糖酶、纤维素酶、木聚糖酶和木质素过氧化物酶共5种水解酶活性,为评价和合理利用鸟巢菌资源提供依据。结果表明,所有菌株均具有以上水解酶活性,同一种水解酶的活性在不同菌株之间以及同一菌株的不同水解酶活性差异显著。其中红蛋巢菌134表现出最大的几丁质酶活性,达到122.1 U/mL;黑蛋巢菌24的β-1,3-葡聚糖酶活性最强,达到143.7 U/mL;在25℃下培养,黑蛋巢菌185的纤维素酶活性最高,达到62.3 U/mL,35℃下培养,菌株185的纤维素酶活性也最高,达到了137.6 U/mL,所有菌株的纤维素酶活性在35℃培养4周比25℃培养2周要高;在25℃下培养时,菌株134的木聚糖酶活性最高,达到了464.8 U/mL,35℃培养4周时,菌株185具有最高的木聚糖酶活性,达到了745.6 U/mL,总体上木聚糖酶的活性在35℃培养4周高于25℃培养3周;黑蛋巢菌150表现出最高的木质素过氧化物酶活性,为0.297 U/mL。总体比较,菌株185的复合酶系较发达,有望利用该菌株完成植物秸杆的降解及利用。
通過測定黑蛋巢菌屬9箇菌株和紅蛋巢菌屬6箇菌株的幾丁質酶、β-1,3-葡聚糖酶、纖維素酶、木聚糖酶和木質素過氧化物酶共5種水解酶活性,為評價和閤理利用鳥巢菌資源提供依據。結果錶明,所有菌株均具有以上水解酶活性,同一種水解酶的活性在不同菌株之間以及同一菌株的不同水解酶活性差異顯著。其中紅蛋巢菌134錶現齣最大的幾丁質酶活性,達到122.1 U/mL;黑蛋巢菌24的β-1,3-葡聚糖酶活性最彊,達到143.7 U/mL;在25℃下培養,黑蛋巢菌185的纖維素酶活性最高,達到62.3 U/mL,35℃下培養,菌株185的纖維素酶活性也最高,達到瞭137.6 U/mL,所有菌株的纖維素酶活性在35℃培養4週比25℃培養2週要高;在25℃下培養時,菌株134的木聚糖酶活性最高,達到瞭464.8 U/mL,35℃培養4週時,菌株185具有最高的木聚糖酶活性,達到瞭745.6 U/mL,總體上木聚糖酶的活性在35℃培養4週高于25℃培養3週;黑蛋巢菌150錶現齣最高的木質素過氧化物酶活性,為0.297 U/mL。總體比較,菌株185的複閤酶繫較髮達,有望利用該菌株完成植物秸桿的降解及利用。
통과측정흑단소균속9개균주화홍단소균속6개균주적궤정질매、β-1,3-포취당매、섬유소매、목취당매화목질소과양화물매공5충수해매활성,위평개화합리이용조소균자원제공의거。결과표명,소유균주균구유이상수해매활성,동일충수해매적활성재불동균주지간이급동일균주적불동수해매활성차이현저。기중홍단소균134표현출최대적궤정질매활성,체도122.1 U/mL;흑단소균24적β-1,3-포취당매활성최강,체도143.7 U/mL;재25℃하배양,흑단소균185적섬유소매활성최고,체도62.3 U/mL,35℃하배양,균주185적섬유소매활성야최고,체도료137.6 U/mL,소유균주적섬유소매활성재35℃배양4주비25℃배양2주요고;재25℃하배양시,균주134적목취당매활성최고,체도료464.8 U/mL,35℃배양4주시,균주185구유최고적목취당매활성,체도료745.6 U/mL,총체상목취당매적활성재35℃배양4주고우25℃배양3주;흑단소균150표현출최고적목질소과양화물매활성,위0.297 U/mL。총체비교,균주185적복합매계교발체,유망이용해균주완성식물갈간적강해급이용。
We determined the activities of five kinds of hydrolases from nine Cyathus and six Nidula isolates for the proper utilization and evaluation of bird’s nest fungi.Experimental results show that there are significant differences for the same hydrolase activity among different isolates,as well as for the same isolate among different hydrolase activities.Nidula spp.134 shows the highest chitinase activity of 122.1 U/mL.Cyathus spp.24 shows the highest β-1,3-glucanase activity of 143.7 U/mL.Cyathus spp.185,cultured at 25℃,shows the highest cellulase activity of 62.3U/mL.It also shows the highest cellulase activity of 137.6 U/mL when cultured at 35℃.The cellulase activities of all isolates cultured at 35℃ for 4 weeks are higher than those cultured at 25℃ for 2 weeks.Nidula spp.134 shows the highest xylanase activity of 464.8 U/mL when cultured at 25℃.Cyathus spp.185 shows the highest xylanase activity of 745.6 U/mL when cultured at 35℃.The xylanase activity of all isolates cultured at 35℃ for 4 weeks are higher than those cultured at 25℃ for 3 weeks.Cyathus spp.150 shows the highest lignin peroxidase activity of 0.297 U/mL when cultured at 25℃.Cyathus spp.185 shows higher complex hydrolase activities,as compared with other isolates.It may be desirable for the degradation of plant straw.
