CAJ | 학술논문

利用固相萃取-高效液相色谱-串联质谱技术，建立了羊肉中克伦特罗、莱克多巴胺、沙丁胺醇3种β-兴奋剂的测定方法。样品经乙酸铵缓冲溶液提取后，用MCX固相萃取柱净化，以甲酸-乙腈和甲酸-乙酸铵缓冲溶液为流动相，梯度洗脱，采用HPLC—MS／MS电喷雾正离子（ESI^＋）电离，多反应监测（MRM）模式检测，外标法定量。结果表明，3种β-兴奋剂在2-100ng／mL范围内均具有良好的线性关系，相关系数不低于0．9988，加标回收率为91-3％～103.8％，相对标准偏差为2．5％-5．7％。该方法准确、灵敏、高效、环保，适用于羊肉中克伦特罗、莱克多巴胺、沙丁胺醇3种β-兴奋剂的同时测定。
이용고상췌취-고효액상색보-천련질보기술，건립료양육중극륜특라、래극다파알、사정알순3충β-흥강제적측정방법。양품경을산안완충용액제취후，용MCX고상췌취주정화，이갑산-을정화갑산-을산안완충용액위류동상，제도세탈，채용HPLC—MS／MS전분무정리자（ESI^＋）전리，다반응감측（MRM）모식검측，외표법정량。결과표명，3충β-흥강제재2-100ng／mL범위내균구유량호적선성관계，상관계수불저우0．9988，가표회수솔위91-3％～103.8％，상대표준편차위2．5％-5．7％。해방법준학、령민、고효、배보，괄용우양육중극륜특라、래극다파알、사정알순3충β-흥강제적동시측정。
A soild phase extraction-high performance liquid chromatography-tandem mass spectrophy （HPLC-MS/MS）was developed for determination of three β-agonists such as clenbuterol, ractomine, salbutemol residues in mutton. The samples were extracted by ammonium acetate buffer, and purified by MCX solid-phase extraction column. Using Formic aeid-acetonitrile and formic acid-ammonium acetate buffer as mobile phase by gradient elution, the analysis of target compounds was carried out by HPLC-MS/MS under positive electrospray ionization （ESI^＋） and multiple reaction monitoring （MRM）mode. An external standard was employed for quantitative analysis. The calibration curves were in good linearity between the peak area and the concentration of 2 to 100 ng/mL for three β-agonists with correlation coefficients not less than 0.998 8. The spiked recoveries ranged from 91.3% to 103.8% with relative standard deviations of 2.5% to 5.7%. The proposed method is accurate, sensitive and high-efficient ,and is applicable for simultaneous determination of three β-agonists in mutton.