CAJ | 학술논문

建立了番茄中多茵灵和啶虫脒的高效液相色谱-串联质谱（HPLC—MS／MS）测定方法。样品经乙腈提取后．用Sep-pakvac固相萃取柱净化，以乙腈和甲酸-乙酸铵缓冲溶液为流动相，梯度洗脱，采用HPLC-MS／MS电喷雾正离子（ESI＋）电离，多反应监测（MRM）模式检测，基质匹配外标法定量。结果表明，多茵灵和啶虫脒在1-100ng／mL范围内均具有良好的线性关系，相关系数不低于0．9985，平均加标回收率为87．4％-96．8％，相对标准偏差为3．7％-6．9％。该方法具有简单、快速、灵敏度高和定性准确等优点，能够满足多茵灵和啶虫脒残留分析的要求。
건립료번가중다인령화정충미적고효액상색보-천련질보（HPLC—MS／MS）측정방법。양품경을정제취후．용Sep-pakvac고상췌취주정화，이을정화갑산-을산안완충용액위류동상，제도세탈，채용HPLC-MS／MS전분무정리자（ESI＋）전리，다반응감측（MRM）모식검측，기질필배외표법정량。결과표명，다인령화정충미재1-100ng／mL범위내균구유량호적선성관계，상관계수불저우0．9985，평균가표회수솔위87．4％-96．8％，상대표준편차위3．7％-6．9％。해방법구유간단、쾌속、령민도고화정성준학등우점，능구만족다인령화정충미잔류분석적요구。
A high performance liquid chromatography--tandem mass spectrophy（HPLC-MS/MS）was developed for determination of carbendazim and acetamiprid residues in tomato. The samples were extracted by acetonitrile,and purified by Sep-pak vac solid-phase extraction column. Acetonitrile and formic acid-ammonium acetate buffer were used as mobile phase by gradient elution. The analysis of target compounds was carried out by HPLC-MS/MS under positive electrospray ionization （ESI＋）and multiple reaction monitoring （MRM）mode. A matrix matched external standard was used for quantitative analysis. The calibration curves were in good linearity between the peak area and the concentration of 1-100 ng,/mL for carbendazim and acetamiprid with correlation coefficients not less than 0.998 5. The average spiked recoveries ranged from 87,4% to 96.8% with relative standard deviations of 3.7%-6.9%. The proposed method was simple, rapid, sensetive and accurate,it could meet the requirements of residue analysis.